The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Coatomer, Arf1p, and nucleotide are required to bud coat protein complex I-coated vesicles from large synthetic liposomes
Open accessAbstract
Synthetic coat protein complex I (COPI)-coated vesicles form spontaneously from large (≈300 nm in diameter), chemically defined liposomes incubated with coatomer, Arf1p, and guanosine 5′-[γ-thio]triphosphate. Coated vesicles are 40–70 nm in diameter, approximately the size of COPI vesicles formed from native membranes. The formation of COPI-coated buds and vesicles and the binding of Arf1p to donor liposomes depends on guanosine 5′-[γ-thio]triphosphate. In contrast to the behavior of the COPII coat, coatomer binds to liposomes containing a variety of charged or neutral phospholipids. However, the formation of COPI buds and vesicles is stimulated by acidic phospholipids. In the absence of Arf1p, coatomer binds to liposomes containing dioleoylphosphatidic acid as a sole acidic phospholipid to form large coated surfaces without forming COPI-coated buds or vesicles. We conclude that Arf1p-GTP and coatomer comprise the minimum apparatus necessary to create a COPI-coated vesicle.
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Helms, J. B., & Rothman, J. E. (1992) · Nature · doi:10.1038/360352a0 · Cited by 746
Cited by
ARF proteins: roles in membrane traffic and beyond
D'Souza-Schorey, C., & Chavrier, P. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1910 · Cited by 1,378
Schmidt, A., Wolde, M., Thiele, C., Fest, W., Kratzin, H., Podtelejnikov, A. V., Witke, W., Huttner, W. B., & Söling, H.-D. (1999) · Nature · doi:10.1038/43613 · Cited by 533
Springer, S., Spang, A., & Schekman, R. (1999) · Cell · doi:10.1016/s0092-8674(00)80722-9 · Cited by 287
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Metadata
- Authors
- Spang, A., Matsuoka, K., Hamamoto, S., Schekman, R., & Orci, L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1998-09-15
- Volume
- 95
- Issue
- 19
- Pages
- 11199-11204
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2171810148
- Cited by
- 196
- References
- 1
- FWCI
- 6.32
- Type
- journal article
- Language
- en
- Open access
- green
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- Spang, A., Matsuoka, K., Hamamoto, S., Schekman, R., & Orci, L. (1998). Coatomer, Arf1p, and nucleotide are required to bud coat protein complex I-coated vesicles from large synthetic liposomes. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.95.19.11199
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