The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries
Open accessAbstract
Ribosome display was applied for affinity selection of antibody single-chain fragments (scFv) from a diverse library generated from mice immunized with a variant peptide of the transcription factor GCN4 dimerization domain. After three rounds of ribosome display, positive scFvs were isolated and characterized. Several different scFvs were selected, but those in the largest group were closely related to each other and differed in 0 to 5 amino acid residues with respect to their consensus sequence, the likely common progenitor. The best scFv had a dissociation constant of (4 ± 1) × 10−11 M, measured in solution. One amino acid residue in complementarity determining region L1 was found to be responsible for a 65-fold higher affinity than the likely progenitor. It appears that this high-affinity scFv was selected from the mutations occurring during ribosome display in vitro, and that this constitutes an affinity maturation inherent in this method. The in vitro-selected scFvs could be functionally expressed in the Escherichia coli periplasm with good yields or prepared by in vitro refolding. Thus, ribosome display can be a powerful methodology for in vitro library screening and simultaneous sequence evolution.
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Cites
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Self-citationVaughan, T. J., Williams, A. J., Pritchard, K., Osbourn, J. K., Pope, A. R., Earnshaw, J. C., McCafferty, J., Hodits, R. A., Wilton, J., & Johnson, K. S. (1996) · Nature Biotechnology · doi:10.1038/nbt0396-309 · Cited by 1,027
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The role of dynamic conformational ensembles in biomolecular recognition
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Self-citationStability engineering of antibody single-chain Fv fragments
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Self-citationPhage display and other peptide display technologies
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Metadata
- Authors
- Hanes, J., Jermutus, L., Weber-Bornhauser, S., Bosshard, H. R., & Plückthun, A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1998-11-24
- Volume
- 95
- Issue
- 24
- Pages
- 14130-14135
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2056374924
- Cited by
- 290
- References
- 33
- FWCI
- 22.18
- Type
- journal article
- Language
- en
- Open access
- green
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- Hanes, J., Jermutus, L., Weber-Bornhauser, S., Bosshard, H. R., & Plückthun, A. (1998). Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.95.24.14130
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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