The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of d -1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis
Open accessAbstract
For many years it was accepted that isopentenyl diphosphate, the common precursor of all isoprenoids, was synthesized through the well known acetate/mevalonate pathway. However, recent studies have shown that some bacteria, including Escherichia coli , use a mevalonate-independent pathway for the synthesis of isopentenyl diphosphate. The occurrence of this alternative pathway has also been reported in green algae and higher plants. The first reaction of this pathway consists of the condensation of (hydroxyethyl)thiamin derived from pyruvate with the C1 aldehyde group of d -glyceraldehyde 3-phosphate to yield d -1-deoxyxylulose 5-phosphate. In E. coli , d -1-deoxyxylulose 5-phosphate is also a precursor for the biosynthesis of thiamin and pyridoxol. Here we report the molecular cloning and characterization of a gene from E. coli , designated dxs, that encodes d -1-deoxyxylulose-5-phosphate synthase. The dxs gene was identified as part of an operon that also contains ispA , the gene that encodes farnesyl-diphosphate synthase. d -1-Deoxyxylulose-5-phosphate synthase belongs to a family of transketolase-like proteins that are highly conserved in evolution.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
[19] Rapid and efficient site-specific mutagenesis without phenotypic selection
Kunkel, T. A., Roberts, J. D., & Zakour, R. A. (1987) · Methods in Enzymology · doi:10.1016/0076-6879(87)54085-x · Cited by 5,261
Current protocols in molecular biology
(1988) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(88)90131-4 · Cited by 4,160
Pedigrees of some mutant strains of Escherichia coli K-12
Bachmann, B. J. (1972) · Bacteriological Reviews · doi:10.1128/br.36.4.525-557.1972 · Cited by 1,837
Kohara, Y., Akiyama, K., & Isono, K. (1987) · Cell · doi:10.1016/0092-8674(87)90503-4 · Cited by 1,741
Rohmer, M., Knani, M., Simonin, P., Sutter, B., & Sahm, H. (1993) · Biochemical Journal · doi:10.1042/bj2950517 · Cited by 1,201
Self-citationLichtenthaler, H. K., Schwender, J., Disch, A., & Rohmer, M. (1997) · FEBS Letters · doi:10.1016/s0014-5793(96)01404-4 · Cited by 712
Self-citationRohmer, M., Seemann, M., Horbach, S., Bringer-Meyer, S., & Sahm, H. (1996) · Journal of the American Chemical Society · doi:10.1021/ja9538344 · Cited by 484
Self-citationYouvan, D. C., Bylina, E. J., Alberti, M., Begusch, H., & Hearst, J. E. (1984) · Cell · doi:10.1016/0092-8674(84)90429-x · Cited by 439
Biosynthesis of isoprenoid compounds
(1982) · General Pharmacology: The Vascular System · doi:10.1016/0306-3623(82)90032-5 · Cited by 371
Cited by
Isoprenoid biosynthesis: The evolution of two ancient and distinct pathways across genomes
Lange, B. M., Rujan, T., Martin, W., & Croteau, R. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.240454797 · Cited by 881
Biosynthesis of isoprenoids via the non-mevalonate pathway
Eisenreich, W., Bacher, A., Arigoni, D., & Rohdich, F. (2004) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-004-3381-z · Cited by 668
Estévez, J. M., Cantero, A., Reindl, A., Reichler, S., & León, P. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m100854200 · Cited by 668
Deoxyxylulose phosphate pathway to terpenoids
Eisenreich, W., Rohdich, F., & Bacher, A. (2001) · Trends in Plant Science · doi:10.1016/s1360-1385(00)01812-4 · Cited by 502
Takahashi, S., Kuzuyama, T., Watanabe, H., & Seto, H. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.17.9879 · Cited by 469
Metadata
- Authors
- Lois, L. M., Campos, N., Putra, S. R., Danielsen, K., Rohmer, M., & Boronat, A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1998-03-03
- Volume
- 95
- Issue
- 5
- Pages
- 2105-2110
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2019030973
- Cited by
- 354
- References
- 33
- FWCI
- 11.71
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of d -1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis. https://app.cochat.ai/papers/10.1073/pnas.95.5.2105
- The paper
- Lois, L. M., Campos, N., Putra, S. R., Danielsen, K., Rohmer, M., & Boronat, A. (1998). Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of d -1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.95.5.2105
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
