Inhibition of FtsZ polymerization by SulA, an inhibitor of septation in Escherichia coli
Open accessAbstract
The bacterial cell division protein FtsZ assembles into the cytokinetic Z ring that directs cytokinesis in prokaryotes. In Escherichia coli the formation of the Z ring is prevented by induction of the cell division inhibitor SulA (SfiA), a component of the SOS response. Here we show that a MalE-SulA fusion that retains this inhibitory function in vivo inhibits the GTPase activity and polymerization of FtsZ in vitro . MalE-SulA10, which does not block Z ring formation in vivo , is unable to inhibit the GTPase activity and polymerization in vitro . Furthermore, FtsZ114, which is refractory to SulA in vivo , is not inhibited by MalE-SulA. These results indicate that SulA blocks Z ring formation by blocking FtsZ polymerization.
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SulA of E. coli and other enterobacterial members, and YneA of B. subtilis, inhibit cell division by interfering with FtsZ activities either directly or indirectly.
“SulA of E. coli and other enterobacterial members, and YneA of B. subtilis, inhibit cell division by interfering with the FtsZ activities either directly or indirectly (Huisman and D'Ari, 1981; Bi and Lutkenhaus, 1993; Mukherjee et al., 1998; Cordell et al., 2003; Kawai et al., 2003; Kawai and Ogasawara, 2006).”
From Interference of <i>Mycobacterium tuberculosis</i> cell division by Rv2719c, a cell wall hydrolase (2006) · cites this paper as Mukherjee et al., 1998
The Abstract and Discussion (page numbers unavailable) show that SulA inhibits cell division in E. coli by blocking FtsZ polymerization and Z-ring formation. Our copy does not address SulA in other enterobacteria or YneA in B. subtilis, which are also included in the citing sentence. (LLM, conf=0.87)
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Jourdain, L., Curmi, P., Sobel, A., Pantaloni, D., & Carlier, M.-F. (1997) · Biochemistry · doi:10.1021/bi971491b · Cited by 245 · see citing works
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- Pogliano, J., Pogliano, K., Weiss, D. S., Losick, R. & Beckwith, J. (1997) Proc. Natl. Acad. Sci. USA 94, 559-564.
- Ma, X., Ehrhardt, D. W. & Margolin, W. (1996) Proc. Natl. Acad. Sci. USA 93, 12998-13003.
- Mukherjee, A., Dai, K. & Lutkenhaus, J. (1993) Proc. Natl. Acad. Sci. USA 90, 1053-1057.
- Erickson, H. P., Taylor, D. W., Taylor, K. A. & Bramhill, D. (1996) Proc. Natl. Acad. Sci. USA 93, 519-523.
- Neidhardt, F. C., Curtiss, R., Ingra- ham, J. L., Lin, E. C. C., Low, K. B., Magasanik, B., Reznikoff, W. S., Riley, M., Schaechter, M. & Umbarger, H. E. (Am. Soc. Microbiol., Washington, DC), pp. 1400-1416.
- Mitzusawa, S. & Gottesman, S. (1983) Proc. Natl. Acad. Sci. USA 80, 358-362.
- Bork, P., Sander, C. & Valencia, A. (1994) Proc. Natl. Acad. Sci. USA 89, 7290-7294.
- Hill, T. M., Sharma, B., Valjavec-Gratian, M. & Smith, J. (1997) J. Bacteriol. 179, 1931-1939. 2890 Biochemistry: Mukherjee et al. Proc. Natl. Acad. Sci. USA 95 (1998)
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- Authors
- Mukherjee, A., Cao, C., & Lutkenhaus, J.
- Affiliations
- University of Kansas Medical Center (US)
- Harvard University (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1998-03-17
- Volume
- 95
- Issue
- 6
- Pages
- 2885-2890
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC19664
- OpenAlex
- W2018163512
- Cited by
- 247
- References
- 1
- Citations by year
- 2026: 13 · 2025: 8 · 2024: 5 · 2023: 11 · 2022: 9 · 2021: 6 · 2020: 11 · 2019: 14 · 2018: 8 · 2017: 9 · 2016: 9 · 2015: 10 · 2014: 15 · 2013: 6 · 2012: 11
- FWCI
- 4.01
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of General Medical Sciences (R37GM029764, GM 29764, R01GM029764)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Keywords
- FtsZ, Sula, FtsZ polymerization, Z-ring formation, bacterial cell division, cytokinesis inhibition, GTPase activity, SOS response, Escherichia coli
- MeSH terms
- Bacterial Proteins, Carrier Proteins, Cell Division, Cytoskeletal Proteins, Escherichia coli, Monosaccharide Transport Proteins, Protein Binding, Recombinant Fusion Proteins, ATP-Binding Cassette Transporters, GTP-Binding Proteins, GTP Phosphohydrolases, Escherichia coli Proteins, Periplasmic Binding Proteins, Maltose-Binding Proteins
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