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Highly sensitive biological and chemical sensors based on reversible fluorescence quenching in a conjugated polymer
Open accessAbstract
The fluorescence of a polyanionic conjugated polymer can be quenched by extremely low concentrations of cationic electron acceptors in aqueous solutions. We report a greater than million-fold amplification of the sensitivity to fluorescence quenching compared with corresponding “molecular excited states.” Using a combination of steady-state and ultrafast spectroscopy, we have established that the dramatic quenching results from weak complex formation [polymer (−) /quencher (+) ], followed by ultrafast electron transfer from excitations on the entire polymer chain to the quencher, with a time constant of 650 fs. Because of the weak complex formation, the quenching can be selectively reversed by using a quencher-recognition diad. We have constructed such a diad and demonstrate that the fluorescence is fully recovered on binding between the recognition site and a specific analyte protein. In both solutions and thin films, this reversible fluorescence quenching provides the basis for a new class of highly sensitive biological and chemical sensors.
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Cited by
Self-assembled π-stacks of functional dyes in solution: structural and thermodynamic features
Chen, Z., Lohr, A., Saha-Möller, C. R., & Würthner, F. (2009) · Chem. Soc. Rev. · doi:10.1039/b809359h · Cited by 1,051
Hou, J., Park, M.-H., Zhang, S., Yao, Y., Chen, L.-M., Li, J.-H., & Yang, Y. (2008) · Macromolecules · doi:10.1021/ma800820r · Cited by 760
Self-citationConjugated Polyelectrolytes: Synthesis, Photophysics, and Applications
Jiang, H., Taranekar, P., Reynolds, J. R., & Schanze, K. S. (2009) · Angewandte Chemie International Edition · doi:10.1002/anie.200805456 · Cited by 734
Novel Electroluminescent Conjugated Polyelectrolytes Based on Polyfluorene
Huang, F., Wu, H., Wang, D., Yang, W., & Cao, Y. (2004) · Chemistry of Materials · doi:10.1021/cm034650o · Cited by 632
Single chain spectroscopy of conformational dependence of conjugated polymer photophysics
Huser, T., Yan, M., & Rothberg, L. J. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.21.11187 · Cited by 322
Metadata
- Authors
- Chen, L., McBranch, D. W., Wang, H.-L., Helgeson, R., Wudl, F., & Whitten, D. G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-10-26
- Volume
- 96
- Issue
- 22
- Pages
- 12287-12292
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2135437943
- Cited by
- 857
- References
- 0
- FWCI
- 10.78
- Type
- journal article
- Language
- en
- Open access
- green
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- Chen, L., McBranch, D. W., Wang, H.-L., Helgeson, R., Wudl, F., & Whitten, D. G. (1999). Highly sensitive biological and chemical sensors based on reversible fluorescence quenching in a conjugated polymer. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.96.22.12287
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