The DOI matches its Crossref record.
A remarkable, precisely timed release of hyperglycemic hormone from endocrine cells in the gut is associated with ecdysis in the crab Carcinus maenas
Open accessAbstract
Molting or ecdysis is the most fundamentally important process in arthropod life history, because shedding of the exoskeleton is an absolute prerequisite for growth and metamorphosis. Although the hormonal mechanisms driving ecdysis in insects have been studied extensively, nothing is known about these processes in crustaceans. During late premolt and during ecdysis in the crab Carcinus maenas, we observed a precise and reproducible surge in hemolymph hyperglycemic hormone (CHH) levels, which was over 100-fold greater than levels seen in intermolt animals. The source of this hormone surge was not from the eyestalk neurosecretory tissues but from previously undescribed endocrine cells (paraneurons), in defined areas of the foregut and hindgut. During premolt (the only time when CHH is expressed by these tissues), the gut is the largest endocrine tissue in the crab. The CHH surge, which is a result of an unusual, almost complete discharge of the contents of the gut endocrine cell, regulates water and ion uptake during molting, thus allowing the swelling necessary for successful ecdysis and the subsequent increase in size during postmolt. This study defines an endocrine brain/gut axis in the arthropods. We propose that the ionoregulatory process controlled by CHH may be common to arthropods, in that, for insects, a similar mechanism seems to be involved in antidiuresis. It also seems likely that a cascade of very precisely coordinated release of (neuro) hormones controls ecdysis…
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Haemolymph content will be increased to unfold the exoskeleton and maintain the shape before the endocuticle form to harden the exoskeleton during the intermoult phase (Chung et al., 1999).”
From HORSESHOE CRAB AND ITS SPAWNING GROUND CONDITION IN JOHOR LAMA, JOHOR (2022) · cites this paper as Chung et al., 1999
In the citing paper
“Horseshoe crab larvae exoskeleton are soft and malleable during the initial period of posthatch and post-ecdysis.”
From HORSESHOE CRAB AND ITS SPAWNING GROUND CONDITION IN JOHOR LAMA, JOHOR (2022) · cites this paper as Chung et al., 1999
Citations
Cites
No references could be resolved yet.
Research works printed but not linked: 32
- 10.1126/science.271.5245.88 · DOI
- 10.1126/science.271.5245.40 · DOI
- 10.1523/JNEUROSCI.17-11-04389.1997 · DOI
- 10.1242/jeb.200.5.869 · DOI
- 10.1242/jeb.200.24.3245 · DOI
- 10.1242/jeb.202.4.343 · DOI
- 10.1016/S0065-2806(08)60144-7 · DOI
- 10.1007/BF01928162 · DOI
- F Van Herp Advances in Arthropod Endocrinology, eds G M Coast, S G Webster (Cambridge Univ. Press, Cambridge, U.K.), pp. 53–70 (1998).
- 10.1111/j.1432-1033.1996.0358h.x · DOI
- 10.1002/(SICI)1520-6327(1996)32:3/4<375::AID-ARCH10>3.0.CO;2-9 · DOI
- P Drach, C Tchernigovtzeff Vie Milieu Ser A 18, 595–607 (1967).
- 10.1242/jeb.199.7.1579 · DOI
- 10.1007/BF00327278 · DOI
- 10.1016/0014-5793(89)81778-8 · DOI
- 10.1016/0196-9781(91)90119-A · DOI
- D M Maynard, M R Dando Philos Trans R Soc London 268, 162–219 (1974).
- 10.1007/BF00218870 · DOI
- 10.1002/arch.940220110 · DOI
- 10.1007/978-4-431-68066-6 · DOI
- 10.1007/BF00994085 · DOI
- 10.1007/BF00224359 · DOI
- 10.1242/jeb.84.1.89 · DOI
- 10.1242/jeb.70.1.231 · DOI
- 10.1242/jeb.173.1.275 · DOI
- 10.1093/icb/35.6.503 · DOI
- 10.1242/jeb.199.5.1053 · DOI
- 10.1242/jeb.202.7.817 · DOI
- 10.1073/pnas.84.2.575 · DOI
- 10.1163/156854295X00537 · DOI
- Phlippen M. K. Webster S. G. Chung J. S. & Dircksen H. (1999) J. Exp. Biol. in press.
- 10.1016/S0960-9822(09)00439-4 · DOI
Cited by
Sorted by citations
Stentiford, G., & Shields, J. (2005) · Diseases of Aquatic Organisms · doi:10.3354/dao066047 · Cited by 244
HORSESHOE CRAB AND ITS SPAWNING GROUND CONDITION IN JOHOR LAMA, JOHOR
Md Razak, M. R., Kassim, Z., Abd Wahab, R., & Hazahari, N. Y. (2022) · Malaysian Journal of Science · doi:10.22452/mjs.vol41no1.1 · Cited by 4
Metadata
- Authors
- Chung, J. S., Dircksen, H., & Webster, S. G.
- Affiliations
- University of Bonn (DE)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1999-11-09
- Volume
- 96
- Issue
- 23
- Pages
- 13103-13107
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC23907
- OpenAlex
- W2095866501
- Cited by
- 236
- References
- 0
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 9 · 2023: 5 · 2022: 8 · 2021: 9 · 2020: 8 · 2019: 8 · 2018: 7 · 2017: 3 · 2016: 12 · 2015: 10 · 2014: 14 · 2013: 7 · 2012: 12
- FWCI
- 12.89
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Environmental Science › Ecology › Crustacean biology and ecology
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Keywords
- Carcinus maenas, hyperglycemic hormone, molting, gastrointestinal endocrine cells, paraneurons, foregut, hindgut, ion regulation, gut-brain axis, crustacean endocrinology, premolt, antidiuresis
- MeSH terms
- Animals, Base Sequence, Chromatography, High Pressure Liquid, Brachyura, Endocrine Glands, Immunohistochemistry, Invertebrate Hormones, Nerve Tissue Proteins, Radioimmunoassay, Mass Spectrometry, DNA Primers, Molting, Arthropod Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A remarkable, precisely timed release of hyperglycemic hormone from endocrine cells in the gut is associated with ecdysis in the crab Carcinus maenas. https://app.cochat.ai/papers/10.1073/pnas.96.23.13103
- The paper
- Chung, J. S., Dircksen, H., & Webster, S. G. (1999). A remarkable, precisely timed release of hyperglycemic hormone from endocrine cells in the gut is associated with ecdysis in the crab Carcinus maenas. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.96.23.13103
