The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Comparative genomic hybridization, loss of heterozygosity, and DNA sequence analysis of single cells
Open accessAbstract
A PCR strategy is described for global amplification of DNA from a single eukaryotic cell that enables the comprehensive analysis of the whole genome. By comparative genomic hybridization, not only gross DNA copy number variations, such as monosomic X and trisomic 21 in single male cells and cells from Down’s syndrome patients, respectively, but multiple deletions and amplifications characteristic for human tumor cells are reliably retrieved. As a model of heterogeneous cell populations exposed to selective pressure, we have studied single micrometastatic cells isolated from bone marrow of cancer patients. The observed congruent pattern of comparative genomic hybridization data, loss of heterozygosity, and mutations as detected by sequencing attests to the technique’s fidelity and demonstrates its usefulness for assessing clonal evolution of genetic variants in complex populations.
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Cites
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Cited by
Tumor heterogeneity: Causes and consequences
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Eleven grand challenges in single-cell data science
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Comprehensive human genome amplification using multiple displacement amplification
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Single-cell sequencing-based technologies will revolutionize whole-organism science
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Detection, clinical relevance and specific biological properties of disseminating tumour cells
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Self-citation
Metadata
- Authors
- Klein, C. A., Schmidt-Kittler, O., Schardt, J. A., Pantel, K., Speicher, M. R., & Riethmüller, G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-04-13
- Volume
- 96
- Issue
- 8
- Pages
- 4494-4499
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1989215251
- Cited by
- 450
- References
- 27
- FWCI
- 10.64
- Type
- journal article
- Language
- en
- Open access
- green
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- Klein, C. A., Schmidt-Kittler, O., Schardt, J. A., Pantel, K., Speicher, M. R., & Riethmüller, G. (1999). Comparative genomic hybridization, loss of heterozygosity, and DNA sequence analysis of single cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.96.8.4494
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