The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Escherichia coli CspA-family RNA chaperones are transcription antiterminators
Open accessAbstract
CspA, the major cold-shock protein of Escherichia coli , is an RNA chaperone, which is thought to facilitate translation at low temperature by destabilizing mRNA structures. Here we demonstrate that CspA, as well as homologous RNA chaperones CspE and CspC, are transcription antiterminators. In vitro , the addition of physiological concentrations of recombinant CspA, CspE, or CspC decreased transcription termination at several intrinsic terminators and also decreased transcription pausing. In vivo , overexpression of cloned CspC and CspE at 37°C was sufficient to induce transcription of the metY - rpsO operon genes nusA , infB , rbfA , and pnp located downstream of multiple transcription terminators. Similar induction of downstream metY - rpsO operon genes was observed at cold shock, a condition to which the cell responds by massive overproduction of CspA. The products of nusA , infB , rbfA , and pnp —NusA, IF2, RbfA, and PNP—are known to be induced at cold shock. We propose that the cold-shock induction of nusA , infB , rbfA , and pnp occurs through transcription antitermination, which is mediated by CspA and other cold shock-induced Csp proteins.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Molecular cloning: A laboratory manual
Deininger, P. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,682
PCR protocols — A guide to methods and applications
U.G. (1990) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(90)90252-7 · Cited by 11,702
Escherichia coli and Salmonella typhimurium: Cellular and molecular biology
Lejeune, P. (1988) · Biochimie · doi:10.1016/0300-9084(88)90119-8 · Cited by 4,072
CspA, the Major Cold-shock Protein of Escherichia coli, Is an RNA Chaperone
Jiang, W., Hou, Y., & Inouye, M. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.1.196 · Cited by 649
Self-citationMajor cold shock protein of Escherichia coli.
Goldstein, J., Pollitt, N. S., & Inouye, M. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.1.283 · Cited by 542
Self-citationBASIC MECHANISMS OF TRANSCRIPT ELONGATION AND ITS REGULATION
Uptain, S. M., Kane, C. M., & Chamberlin, M. J. (1997) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.66.1.117 · Cited by 445
The cold‐shock response — a hot topic
Jones, P. G., & Inouye, M. (1994) · Molecular Microbiology · doi:10.1111/j.1365-2958.1994.tb00359.x · Cited by 440
Self-citationWolffe, A. P. (1994) · BioEssays · doi:10.1002/bies.950160407 · Cited by 381
Protein-protein communication within the transcription apparatus
Ishihama, A. (1993) · Journal of Bacteriology · doi:10.1128/jb.175.9.2483-2489.1993 · Cited by 360
Igarashi, K., & Ishihama, A. (1991) · Cell · doi:10.1016/0092-8674(91)90553-b · Cited by 342
Cited by
Stress responses in lactic acid bacteria
van de Guchte, M., Serror, P., Chervaux, C., Smokvina, T., Ehrlich, S. D., & Maguin, E. (2002) · Antonie van Leeuwenhoek · doi:10.1023/a:1020631532202 · Cited by 648
Initiation of Protein Synthesis in Bacteria
Laursen, B. S., Sørensen, H. P., Mortensen, K. K., & Sperling-Petersen, H. U. (2005) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.69.1.101-123.2005 · Cited by 616
Transcriptional and Post-transcriptional Control of Cold-shock Genes
Gualerzi, C. O., Maria Giuliodori, A., & Pon, C. L. (2003) · Journal of Molecular Biology · doi:10.1016/s0022-2836(03)00732-0 · Cited by 287
Hemm, L., Lichtenberg, E., Tholen, S., Reimann, V., Kakazu, K., Machida, S., Mahbub, M., Schilling, O., Wilde, A., Watanabe, S., et al. (2025) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2506275122 · Cited by 13
Metadata
- Authors
- Bae, W., Xia, B., Inouye, M., & Severinov, K.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2000-07-05
- Volume
- 97
- Issue
- 14
- Pages
- 7784-7789
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2015591844
- Cited by
- 386
- References
- 24
- FWCI
- 10.57
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Escherichia coli CspA-family RNA chaperones are transcription antiterminators. https://app.cochat.ai/papers/10.1073/pnas.97.14.7784
- The paper
- Bae, W., Xia, B., Inouye, M., & Severinov, K. (2000). Escherichia coli CspA-family RNA chaperones are transcription antiterminators. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.97.14.7784
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
