Myosin-V stepping kinetics: A molecular model for processivity
Open accessAbstract
Myosin-V is a molecular motor that moves processively along its actin track. We have used a feedback-enhanced optical trap to examine the stepping kinetics of this movement. By analyzing the distribution of time periods separating discrete approximately 36-nm mechanical steps, we characterize the number and duration of rate-limiting biochemical transitions preceding each such step. These data show that myosin-V is a tightly coupled motor whose cycle time is limited by ADP release. On the basis of these results, we propose a model for myosin-V processivity.
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Other molecular motors, such as myosins V and VI, are processive.
“Other molecular motors, such as myosins V and VI are also processive (34-36”
From Stepping and Stretching (2003) · cites this paper as [35]
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References 28 · Cited by 402
- Authors
- Rief, M., Rock, R. S., Mehta, A. D., Mooseker, M. S., Cheney, R. E., & Spudich, J. A.
- Affiliations
- University of North Carolina at Chapel Hill (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2000-08-15
- Volume
- 97
- Issue
- 17
- Pages
- 9482-9486
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC16890
- OpenAlex
- W2055870007
- Cited by
- 402
- References
- 28
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 7 · 2023: 3 · 2022: 12 · 2021: 3 · 2020: 9 · 2019: 6 · 2018: 10 · 2017: 7 · 2016: 9 · 2015: 13 · 2014: 16 · 2013: 9 · 2012: 15
- FWCI
- 9.07
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Carolina Digital Repository (University of North Carolina at Chapel Hill) (submittedVersion)
- UNC Libraries
- Funders
- National Institute on Deafness and Other Communication Disorders (R01DC003299, R29DC003299, DC03299)
- National Institute of General Medical Sciences (R01GM033289, GM33289)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- myosin V, molecular motors, processivity, actin, ADP release, optical tweezers
- MeSH terms
- Actins, Adenosine Diphosphate, Adenosine Triphosphate, Animals, Brain, Calmodulin-Binding Proteins, Chickens, Feedback, Kinetics, Lasers, Microspheres, Models, Biological, Movement, Nerve Tissue Proteins, Molecular Motor Proteins, Myosin Type V
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