The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Amplification of the neu/erbB-2 oncogene in a mouse model of mammary tumorigenesis
Open accessAbstract
The neu (c-erbB-2, Her-2) protooncogene is amplified and overexpressed in 20-30% of human breast cancers. Although transgenic mouse models have illustrated the role of Neu in the induction of mammary tumors, Neu expression in these models is driven by a strong viral promoter of questionable relevance to the human disease. To ascertain whether expression of activated Neu under the control of the endogenous promoter in the mammary gland could induce mammary tumors we have generated mice that conditionally express activated Neu under the transcriptional control of the intact endogenous Neu promoter. Expression of oncogenic neu in the mammary gland resulted in accelerated lobulo-alveolar development and formation of focal mammary tumors after a long latency period. However, expression of activated Neu under the normal transcriptional control of the endogenous promoter was not sufficient for the initiation of mammary carcinogenesis. Strikingly, all mammary tumors bear amplified copies (2-22 copies) of the activated neu allele relative to the wild-type allele and express highly elevated levels of neu transcript and protein. Thus, like human erbB-2-positive breast tumors, mammary tumorigenesis in this mouse model requires the amplification and commensurate elevated expression of the neu gene.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Consistent with its oncogenic activity, overexpression of wild-type Neu or HER2 under the control of a mammary-specific promoter leads to metastatic mammary tumors in transgenic mice (Andrechek et al., 2000; Finkle et al., 2004).”
From ERBB Receptors: From Oncogene Discovery to Basic Science to Mechanism-Based Cancer Therapeutics (2014) · cites this paper as Andrechek et al., 2000
Citations
Cites
Slamon, D. J., Clark, G. M., Wong, S. G., Levin, W. J., Ullrich, A., & McGuire, W. L. (1987) · Science · doi:10.1126/science.3798106 · Cited by 11,760
Studies of the HER-2/ neu Proto-Oncogene in Human Breast and Ovarian Cancer
Slamon, D. J., Godolphin, W. J., Jones, L. A., Holt, J. A., Wong, S., Keith, D. E., Levin, W. J., Stuart, S. G., Udove, J. A., Ullrich, A., et al. (1989) · Science · doi:10.1126/science.2470152 · Cited by 6,817
Ullrich, A., Coussens, L. M., Hayflick, J. S., Dull, T. J., Gray, A., Tam, A. W., Lee, J., Yarden, Y., Libermann, T. A., Schlessinger, J., et al. (1984) · Nature · doi:10.1038/309418a0 · Cited by 2,751
Coussens, L. M., Yang‐Feng, T. L., Liao, Y.-C., Chen, E., Gray, A., McGrath, J. P., Seeburg, P. H., Libermann, T. A., Schlessinger, J., Francke, U., et al. (1985) · Science · doi:10.1126/science.2999974 · Cited by 1,815
Guy, C. T., Cardiff, R., & Muller, W. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.3.954-961.1992 · Cited by 1,710
Self-citationGuy, C. T., Cardiff, R. D., & Muller, W. J. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.3.954 · Cited by 1,523
Requirement for neuregulin receptor erbB2 in neural and cardiac development
Lee, K., Simon, H. H., Chen, H., Bates, B., Hung, M., & Hauser, C. (1995) · Nature · doi:10.1038/378394a0 · Cited by 1,291
Muller, W., Sinn, E., Pattengale, P., Wallace, R., & Leder, P. (1988) · Cell · doi:10.1016/0092-8674(88)90184-5 · Cited by 1,246
Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor
Yamamoto, T. S., Ikawa, S., Akiyama, T., Semba, K., Nomura, N., Miyajima, N., Saito, T., & Toyoshima, K. (1986) · Nature · doi:10.1038/319230a0 · Cited by 1,204
Guy, C. T., Webster, M. A., Schaller, M. D., Parsons, T. J., Cardiff, R. D., & Muller, W. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.22.10578 · Cited by 1,193
Research works printed but not linked: 4
- W C Dougall, X Qian, N C Peterson, M J Miller, A Samanta, M I Greene Oncogene 9, 2109–2123 (1994).
- N E Hynes, D F Stern Biochim Biophys Acta 1198, 165–184 (1994).
- R Zeillinger, F Kury, K Czerwenka, E Kubista, G Sliutz, W Knogler, J Huber, C Zielinski, G Reiner, R Jakesz Oncogene 4, 109–114 (1989).
- T Ishikawa, M Kobayashi, M Mai, T Suzuki, A Ooi Am J Pathol 151, 761–768 (1997).
Cited by
Sorted by citations
Items 201–230 of 230
Mouse Models of Breast Cancer: Deceptions that Reveal the Truth
Couto, J. P., & Bentires‐Alj, M. (2017) · Springer eBooks · doi:10.1007/978-3-319-48848-6_6 · Cited by 1
Mouse Models of Receptor Tyrosine Kinases
Holcmann, M., Amberg, N., Drobits, B., Glitzner, E., Komposch, K., Robson, J., Savarese, F., Srivatsa, S., Stulnig, G., & Sibilia, M. C. (2014) · Springer eBooks · doi:10.1007/978-1-4939-2053-2_11 · Cited by 1
Garland, B., Delisle, S., Abou-Hamad, J., de Souza, C. D., Zuccarini, R., Cook, D. P., Auer, R., & Sabourin, L. A. (2026) · bioRxiv · doi:10.1101/2025.08.26.672423
Dillon, R. L., & Muller, W. J. (2009) · Humana Press eBooks · doi:10.1007/978-1-60327-945-1_7
Pan, D., Leal, M. D. P. C., Bisarò, B., Tornillo, G., Repetto, D., Pincini, A., Sharma, N., Grasso, S., Turco, E., Cabodi, S., et al. (2011) · InTech eBooks · doi:10.5772/22502
ERalpha and ErbB-2 Cross-talk in Mammary Tumorigenesis and Metastasis
Muller, W. J. (2006) · doi:10.21236/ada463399
Sau, A. (2015) · uO Research (University of Ottawa) · doi:10.20381/ruor-4050
HER-2 as a Prognostic and Predictive Biomarker in Cancer
Zimmer, A. S., Wedamb, S. B., & Lipkowitza, S. (2020) · doi:10.1201/9781003159469-4
Use of genetically engineered mouse models in preclinical drug development
Creedon, H. (2015) · ERA
The role of mTORC2 in mammary morphogenesis and HER2-mediated tumorigenesis
Morrison, M. M. (2015)
Primary and Secondary Events in Oncogene-Driven Tumor Development
Herzig, M., & Christofori, G. (2003) · Humana Press eBooks · doi:10.1007/978-1-59259-313-2_13
HER-2 as a Prognostic and Predictive Biomarker in Cancer
Wedam, S. B., & Lipkowitz, S. (2013) · Pan Stanford Publishing eBooks · doi:10.1201/b15029-5
HER2-Positive (HER2 +) Breast Cancer
Dilday, T., & Yeh, E. S. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-820472-6.00055-4
To, B., Broeker, C. D., Jhan, J.-R., Rempel, R. E., Rennhack, J. P., Hollern, D. P., Jackson, L., Judah, D., Swiatnicki, M., Bylett, E., et al. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.09.24.461727
The role of NEDD9 in HER2-driven breast cancer.
Purazo, M. L. (2023) · doi:10.33915/etd.11996
Mammary Alveolar Proliferation and Differentiation During Early Pregnancy is Regulated by E2F5Preprint
Vusich, J., Garcia-Lerena, J., Corfixsen, R., Talib, R., Atkins, M., Bhurke, A. V., Arora, R., & Andrechek, E. R. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.11.27.625731
Yavaş, Ö., Yavaş, S. E., & Sönmez, G. (2025) · Journal of Molecular Histology · doi:10.1007/s10735-025-10501-8
Neuronal innervation of breast cancer promotes metastatic disseminationPreprint
Schulte, A. J., & Andrechek, E. R. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.11.25.690523
Genomic and functional profiling of gastric cancer
Myllykangas, S. (2008) · Työväentutkimus Vuosikirja
Raghuram, N., Temel, E. I., Kawamata, T., Kozma, K. J., Loch, A. J., Wang, W., Adams, J. R., Muller, W. J., & Egan, S. E. (2024) · Figshare · doi:10.6084/m9.figshare.25918997
Schulte, A., & Andrechek, E. R. (2026) · Journal of Mammary Gland Biology and Neoplasia · doi:10.1007/s10911-026-09599-7 · Cited by 1
Régulation de l'invasion cellulaire induite par les tyrosine kinases dans le cancer du sein
Chevalier, C. (2014) · theses.fr (ABES) · doi:10.70675/692c8de1za82az4e5ez953bz5e90670eb75d
Garland, B., Delisle, S., Abou-Hamad, J., de Souza, C. D., Zuccarini, R., Cook, D. P., Auer, R. C., & Sabourin, L. A. (2026) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.2520063123
neu/erbB-2oncogene in mouse mammary tumorigenesis
Howard, B. A. (2000) · Breast Cancer Research · doi:10.1186/bcr-2000-66673
Metadata
- Authors
- Andrechek, E. R., Hardy, W. R., Siegel, P. M., Rudnicki, M. A., Cardiff, R. D., & Muller, W. J.
- Affiliations
- University of California, Davis (US)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2000-03-14
- Volume
- 97
- Issue
- 7
- Pages
- 3444-3449
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC16259
- OpenAlex
- W2041576667
- Cited by
- 230
- References
- 38
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 5 · 2023: 7 · 2022: 2 · 2021: 6 · 2020: 10 · 2019: 7 · 2018: 6 · 2017: 7 · 2016: 8 · 2015: 9 · 2014: 12 · 2013: 9 · 2012: 8
- FWCI
- 5.59
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (U01CA105490)
- Topics
- Health Sciences › Medicine › Oncology › HER2/EGFR in Cancer Research
- Health Sciences › Medicine › Pathology and Forensic Medicine › Breast Lesions and Carcinomas
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Keywords
- neu oncogene, c-erbB-2, HER2, gene amplification, mammary carcinogenesis, transgenic mouse model, conditional gene expression, endogenous promoter, breast cancer, oncogene overexpression
- MeSH terms
- Animals, Disease Models, Animal, Gene Amplification, Humans, Immunohistochemistry, Mammary Neoplasms, Experimental, Mice, Transgenic, DNA, Complementary, Genes, erbB-2, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
