The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Genetic transformation of HeLa cells by Agrobacterium
Open accessAbstract
Agrobacterium tumefaciens is a soil phytopathogen that elicits neoplastic growths on the host plant species. In nature, however, Agrobacterium also may encounter organisms belonging to other kingdoms such as insects and animals that feed on the infected plants. Can Agrobacterium , then, also infect animal cells? Here, we report that Agrobacterium attaches to and genetically transforms several types of human cells. In stably transformed HeLa cells, the integration event occurred at the right border of the tumor-inducing plasmid's transferred-DNA (T-DNA), suggesting bona fide T-DNA transfer and lending support to the notion that Agrobacterium transforms human cells by a mechanism similar to that which it uses for transformation of plants cells. Collectively, our results suggest that Agrobacterium can transport its T-DNA to human cells and integrate it into their genome.
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Cites
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Agrobacterium -Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool
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Agrobacterium-Mediated Plant Transformation: Biology and Applications
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Agrobacterium T-DNA integration: molecules and models
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Self-citationFunctions of ROL genes in plant secondary metabolism.
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Ancient horizontal gene transfer
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Metadata
- Authors
- Kunik, T., Tzfira, T., Kapulnik, Y., Gafni, Y., Dingwall, C., & Citovsky, V.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2001-01-30
- Volume
- 98
- Issue
- 4
- Pages
- 1871-1876
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2024977774
- Cited by
- 302
- References
- 30
- FWCI
- 29.58
- Type
- journal article
- Language
- en
- Open access
- green
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- Kunik, T., Tzfira, T., Kapulnik, Y., Gafni, Y., Dingwall, C., & Citovsky, V. (2001). Genetic transformation of HeLa cells by Agrobacterium. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.98.4.1871
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