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Overproduction and Physical Characterization of SoxR, a [2Fe-2S] Protein That Governs an Oxidative Response Regulon in Escherichia coli
Open accessAbstract
SoxR protein governs the soxRS (superoxide response) regulon of Escherichia coli by becoming a transcriptional activator when the cells are exposed to compounds that mediate univalent redox reactions, many of which produce superoxide as a by-product. SoxR was overproduced and purified to near homogeneity from a strain bearing an expression vector. It could bind specifically to the soxS operator even in the absence of RNA polymerase. The aerobically purified protein, which is readily autooxidized, could activate the transcription of soxS DNA even without exposure to known inducing agents. SoxR is a globular homodimer. It contains one [2Fe-2S] cluster per polypeptide chain, as demonstrated by optical and EPR spectroscopy combined with stoichiometric analysis of iron content, unpaired-electron-spin density, and reduction by dithionite. The protein is active in its oxidized ([2Fe-2S]2+) state. The presence of a prosthetic group capable of univalent redox reactions may help to explain the activation of the regulon in vivo by compounds that can mediate such reactions.
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Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Molecular cloning: A laboratory manual
Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684
A comprehensive set of sequence analysis programs for the VAX
Devereux, J., Haeberli, P. E., & Smithies, O. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.1part1.387 · Cited by 14,222
Current protocols in molecular biology
Sargent, T. (1988) · Analytical Biochemistry · doi:10.1016/0003-2697(88)90147-9 · Cited by 7,281
[6] Use of T7 RNA polymerase to direct expression of cloned genes
Studier, F. W., Rosenberg, A. H., Dunn, J. J., & Dubendorff, J. W. (1990) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(90)85008-c · Cited by 6,562
Vieira, J., & Messing, J. W. (1982) · Gene · doi:10.1016/0378-1119(82)90015-4 · Cited by 5,984
Improved microfluorometric DNA determination in biological material using 33258 Hoechst
Cesarone, C. F., Bolognesi, C., & Santi, L. (1979) · Analytical Biochemistry · doi:10.1016/0003-2697(79)90131-3 · Cited by 836
Greenberg, J. T., Monach, P. A., Chou, J. H., Josephy, P. D., & Demple, B. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.16.6181 · Cited by 556
The SWISS-PROT protein sequence data bank
Bairoch, A., & Boeckmann, B. (1992) · Nucleic Acids Research · doi:10.1093/nar/20.suppl.2019 · Cited by 530
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Miller, P. F., & Rather, P. N. (2007) · CRC Press eBooks · doi:10.1201/9781420008753.ch3
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Dröge, W., & Hildebrandt, W. (2003) · Oxidative stress and disease · doi:10.1201/9780203912874.ch5
Benyamina, S. M. (2012) · theses.fr (ABES) · doi:10.70675/aafc7ab4z1251z44d8zbdaez0661f78121c0
Metadata
- Authors
- Wu, J., Dunham, W. R., & Weiss, B.
- Affiliations
- University of Michigan (US)
- Corresponding author
- Weiss, Bernard
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1995-04
- Volume
- 270
- Issue
- 17
- Pages
- 10323-10327
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2025874325
- Cited by
- 103
- References
- 35
- Citations by year
- 2025: 2 · 2022: 1 · 2018: 1 · 2017: 2 · 2016: 3 · 2015: 1 · 2014: 5 · 2013: 3 · 2012: 3
- FWCI
- 6.65
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Center for Research Resources (M-01RR00042, M01RR000042)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Metalloenzymes and iron-sulfur proteins
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- SoxR, [2Fe-2S] cluster, SOX, transcriptional activator, Escherichia coli, protein overexpression, protein purification, autooxidation, homodimer, prosthetic groups, superoxide, DNA binding, electron spin resonance
- MeSH terms
- Bacterial Proteins, Base Sequence, Cloning, Molecular, Dithionite, Electron Spin Resonance Spectroscopy, Escherichia coli, Iron-Sulfur Proteins, Metals, Molecular Sequence Data, Oxidation-Reduction, Plasmids, Transcription Factors, Sequence Homology, Amino Acid, DNA Primers, Regulon, Oxidative Stress
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