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mRNA Profiling of Rat Islet Tumors Reveals Nkx 6.1 as a β-Cell-specific Homeodomain Transcription Factor
Open accessAbstract
Development of a high capacity multiplex reverse transcriptase-polymerase chain reaction protocol has allowed us to screen lineage related rat islet tumors classified as alpha-, beta-, and delta-like as judged by their hormone profile for differential expression of more than 50 selected genes. We find that in addition to insulin the insulinoma express the normal beta-cell markers Pdx-1, IAPP, and Glut-2, and that these markers are absent from the glucagonoma: a reflection of the normal alpha-cell. Furthermore, this study suggests that the GLP-1, glucagon, GIP, IGF-1, and insulin receptors as well as E-cadherin, R-cadherin, Id-1, and Id-2 are differentially expressed within the islet of Langerhans. Importantly, insulinoma-specific expression of the recently cloned homeodomain protein Nkx 6.1 predicted beta-cell-specific expression in the normal islet. Immunohistochemistry using antibodies raised against recombinant Nkx 6.1 did indeed localize Nkx 6.1 expression exclusively to the nuclei of normal islet beta-cells. Apart from pancreatic islets only the antral part of the stomach contained Nkx 6.1 mRNA. We conclude that multiplex reverse transcriptase-polymerase chain reaction-based mRNA profiling is a powerful tool to identify differentially expressed genes within phenotypically related cells and propose that Nkx 6.1 is involved in specifying the unique characteristics of the beta-cell.
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Cited by
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Schwitzgebel, V. M., Scheel, D. W., Conners, J. R., Kalamaras, J., Lee, J. E., Anderson, D. J., Sussel, L., Johnson, J. D., & German, M. S. (2000) · Development · doi:10.1242/dev.127.16.3533 · Cited by 678
Sander, M., Neubüser, A., Kalamaras, J., Ee, H. C., Martin, G. R., & German, M. S. (1997) · Genes & Development · doi:10.1101/gad.11.13.1662 · Cited by 587
Gelling, R. W., Du, X. Q., Dichmann, D. S., Rømer, J., Huang, H., Cui, L., Obici, S., Tang, B., Holst, J. J., Fledelius, C., et al. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0237106100 · Cited by 563
Self-citationSvärd, J., Henricson, K. H., Persson-Lek, M., Rozell, B., Lauth, M., Bergström, Å., Ericson, J., Toftgård, R., & Teglund, S. (2006) · Developmental Cell · doi:10.1016/j.devcel.2005.12.013 · Cited by 468
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Hansson, M., Tonning, A., Frandsen, U., Petri, A., Rajagopal, J., Englund, M. C. O., Heller, R. S., Håkansson, J., Fleckner, J., Sköld, H. N., et al. (2004) · Diabetes · doi:10.2337/diabetes.53.10.2603 · Cited by 225
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Metadata
- Authors
- Jensen, J., Serup, P., Karlsen, C., Nielsen, T. F., & Madsen, O. D.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1996-08
- Volume
- 271
- Issue
- 31
- Pages
- 18749-18758
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2055598818
- Cited by
- 210
- References
- 0
- FWCI
- 12.92
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: mRNA Profiling of Rat Islet Tumors Reveals Nkx 6.1 as a β-Cell-specific Homeodomain Transcription Factor. https://app.cochat.ai/papers/10.1074/jbc.271.31.18749
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- Jensen, J., Serup, P., Karlsen, C., Nielsen, T. F., & Madsen, O. D. (1996). mRNA Profiling of Rat Islet Tumors Reveals Nkx 6.1 as a β-Cell-specific Homeodomain Transcription Factor. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.271.31.18749
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