Two Yeast Homologs of ECA39, a Target for c-Myc Regulation, Code for Cytosolic and Mitochondrial Branched-chain Amino Acid Aminotransferases
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ECA39 was isolated as a target gene for c-Myc regulation in mice. We identified two homologs for the murine ECA39 in the yeast Saccharomyces cerevisiae, ECA39 and ECA40, as well as two human homologs. These genes show a significant homology to prokaryotic branched-chain amino acid aminotransferase (BCAT) (EC). To understand the function of eukaryotic ECA39 and ECA40, we deleted either gene from the yeast genome. Activity of branched-chain amino acid aminotransferase was measured in the wild-type and mutants with either leucine, isoleucine, or valine as substrates. The results demonstrate that in S. cerevisiae ECA39 and ECA40 code for mitochondrial and cytosolic branched-chain amino acid aminotransferases, respectively. ECA39 is highly expressed during log phase and is down-regulated during the stationary phase of growth, while ECA40 shows an inverse pattern of gene expression. In agreement with these results, while we previously showed that deletion of ECA39 affected the cell cycle in proliferating cells, we do not observe a growth phenotype in eca40Delta cells. We suggest that BCAT is a target for c-Myc activity and discuss the evolutionary conservation of prokaryotic and eukaryotic BCATs and their possible involvement in regulation of cell proliferation.
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BAT1 is highly expressed during logarithmic growth phase and down-regulated during stationary phase, while BAT2 shows an inverse pattern of expression.
“the BAT1 gene is highly expressed during the logarithmic growth phase and down-regulated during the stationary phase, while the BAT2 gene shows an inverse pattern of expression (Eden et al. 1996).”
From Yeast and bacterial modulation of wine aroma and flavour (2005) · cites this paper as Eden et al. 1996
This transamination reaction is catalysed inside the yeast by mitochondrial and cytosolic branched-chain amino acid aminotransferases encoded by BAT1 and BAT2.
“This reaction is catalysed inside the yeast by mitochondrial and cytosolic branched-chain amino acid aminotransferases encoded by BAT1 and BAT2 (Eden et al. 1996, 2001, Kispal et al. 1996).”
From Yeast and bacterial modulation of wine aroma and flavour (2005) · cites this paper as Eden et al. 1996
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- Authors
- Eden, A., Simchen, G., & Benvenisty, N.
- Affiliations
- Hebrew University of Jerusalem (IL)
- Corresponding author
- Benvenisty, Nissim
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1996-08
- Volume
- 271
- Issue
- 34
- Pages
- 20242-20245
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2061469583
- Cited by
- 116
- References
- 52
- Citations by year
- 2025: 3 · 2023: 5 · 2022: 2 · 2021: 2 · 2020: 7 · 2019: 5 · 2018: 8 · 2017: 3 · 2016: 4 · 2015: 5 · 2014: 7 · 2013: 2 · 2012: 4
- FWCI
- 1.27
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Keywords
- branched-chain amino acid aminotransferase, Saccharomyces cerevisiae, gene deletion, cell cycle regulation, cell proliferation, leucine, isoleucine, valine
- MeSH terms
- Amino Acid Sequence, Transaminases, Animals, Cytoplasm, Fungal Proteins, Genes, Fungal, Humans, Mitochondria, Molecular Sequence Data, Phylogeny, Proteins, RNA, Messenger, Saccharomyces cerevisiae, Signal Transduction, Gene Expression, Proto-Oncogene Proteins c-myc, Sequence Alignment, Sequence Homology, Amino Acid, Mitochondrial Proteins, Saccharomyces cerevisiae Proteins, Mice
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