Effects of Intravesicular H+ and Extracellular H+ and Zn2+ on Insulin Secretion in Pancreatic Beta Cells
Open accessAbstract
The effects of extracellular Zn2+ and pH and intravesicular pH on insulin and 5-hydroxytryptamine (5-HT) secretion from pancreatic beta cells were investigated. Insulin and 5-HT secretion from single cells was detected by amperometry as a series of current spikes corresponding to detection of multimolecular packets secreted by exocytosis. Spike width was used as a measure of the kinetics of clearance from the cell and the area of spikes as a measure of amount released. Changes in extracellular pH from 6.9 to 7.9 caused insulin spikes to become narrower with no change in area, whereas the same treatments had no effect on 5-HT secretion. Treatment of cells with Bafilomycin A1 or N-ethylmaleimide, both of which are expected to increase intravesicular pH by inhibiting V-type H+-ATPase, had no effect on 5-HT secretion but caused insulin spikes to become more narrow. These results indicate that exposure to high pH, whether intravesicular or extracellular, accelerates release of insulin during exocytosis without affecting the amount of insulin released. Increasing extracellular Zn2+ concentration from 0 to 25 microM increased the width and decreased the area of insulin spikes without affecting 5-HT secretion. Zn2+ effects were likely exerted through a common-ion effect on Zn2+-insulin dissociation. It was concluded that intravesicular storage conditions and extracellular ions can affect free insulin concentration in the vicinity of beta cells during secretion.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Carbon fiber amperometry has been successfully applied to rodent β-cells in which the granules have been preloaded with serotonin.
“This technique has been successfully applied to rodent β-cells in which the granules have preloaded with serotonin (36, 74, 634, 663) and modified carbon fibers have been used to detect the release of insulin itself (294).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [36]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Diabetic Hyperglycemia: Link to Impaired Glucose Transport in Pancreatic β Cells
Unger, R. H. (1991) · Science · doi:10.1126/science.2006409 · Cited by 263 · see citing works
Flagged first, then by citations
Research works printed but not linked: 6
- Wightman, R. M., Jankowski, J. A., Kennedy, R. T., Kawagoe, K. T., Schroeder, T. J., Leszczyszyn, D. J., Near, J. A., Diliberto, E. J., and Viveros, O. H. (1991) Proc. Natl. Acad. Sci. U. S. A. 88, 10754 -10758
- Zhou, Z., and Misler, S. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 6938 -6942
- Huang, L., Shen, H., Atkinson, M. A., and Kennedy, R. T. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 608 -612
- Warnock, G. L., Ao, Z., Lakey, J. R. T., and Rajotte, R. V. (1994) in Pancreatic Islet Transplantation Vol I: Procurement of Pancreatic Islets (Lanza, R. P., and Chick, W. L., eds) pp. 81-95, R. G. Landes, Austin, TX 42.
- Bowman, E. J., Siebers, A., and Altendorf, K. (1988) Proc. Natl. Acad. Sci.
- Hazelwood, R. L. (1989) The Endocrine Pancreas Prentice Hall, Englewood Cliffs, NJ
Cited by
Sorted by citations
Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men
Rorsman, P., & Ashcroft, F. M. (2018) · Physiological Reviews · doi:10.1152/physrev.00008.2017 · Cited by 793 · see citing works
- Authors
- Aspinwall, C. A., Brooks, S. A., Kennedy, R. T., & Lakey, J. R. T.
- Affiliations
- University of Florida (US)
- University of Alberta (CA)
- Corresponding author
- Kennedy, Robert T.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1997-12
- Volume
- 272
- Issue
- 50
- Pages
- 31308-31314
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2045267378
- Cited by
- 90
- References
- 1
- Citations by year
- 2025: 2 · 2024: 1 · 2022: 3 · 2021: 4 · 2020: 3 · 2019: 1 · 2017: 2 · 2016: 4 · 2015: 4 · 2014: 2 · 2013: 3 · 2012: 1
- FWCI
- 7.66
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Fondation pour la Recherche Médicale
- National Institutes of Health
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK046960, R0 1DK46960-01)
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
- Keywords
- insulin secretion, pancreatic beta cells, intravesicular pH, extracellular pH, zinc, exocytosis, V-type H+-ATPase, bafilomycin A1, N-ethylmaleimide, common ion effect, amperometry
- MeSH terms
- Insulin Secretion, Animals, Anti-Bacterial Agents, Cells, Cultured, Dogs, Enzyme Inhibitors, Ethylmaleimide, Exocytosis, Proton-Translocating ATPases, Hydrogen, Hydrogen-Ion Concentration, Insulin, Islets of Langerhans, Serotonin, Zinc, Macrolides
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
