The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Identification and Molecular Characterization of an Efflux Pump Involved in Pseudomonas putida S12 Solvent Tolerance
Open accessAbstract
Bacteria able to grow in aqueous:organic two-phase systems have evolved resistance mechanisms to the toxic effects of solvents. One such mechanism is the active efflux of solvents from the cell, preserving the integrity of the cell interior. Pseudomonas putida S12 is resistant to a wide variety of normally detrimental solvents due to the action of such an efflux pump. The genes for this solvent efflux pump were cloned from P. putida S12 and their nucleotide sequence determined. The deduced amino acid sequences encoded by the three genes involved show a striking resemblance to proteins known to be involved in proton-dependent multidrug efflux systems. Transfer of the genes for the solvent efflux pump to solvent-sensitive P. putida strains results in the acquisition of solvent resistance. This opens up the possibilities of using the solvent efflux system to construct bacterial strains capable of performing biocatalytic transformations of insoluble substrates in two-phase aqueous:organic medium.
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Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Cited by
Regulation of bacterial drug export systems
Grkovic, S., Brown, M. H., & Skurray, R. A. (2002) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.66.4.671-701.2002 · Cited by 423
Engineering microbes for tolerance to next-generation biofuels
Dunlop, M. J. (2011) · Biotechnology for Biofuels · doi:10.1186/1754-6834-4-32 · Cited by 293
Mosqueda, G., Ramos-González, M.-I., & Ramos, J. L. (1999) · Gene · doi:10.1016/s0378-1119(99)00113-4 · Cited by 112
Active Efflux of Organic Solvents by Pseudomonas putida S12 Is Induced by Solvents
Kieboom, J., Dennis, J. J., Zylstra, G. J., & de Bont, J. A. M. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.24.6769-6772.1998 · Cited by 104
Self-citationEffect of Organic Solvents on the Yield of Solvent-Tolerant Pseudomonas putida S12
Isken, S., Derks, A., Wolffs, P. F. G., & de Bont, J. A. M. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.6.2631-2635.1999 · Cited by 86
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Metadata
- Authors
- Kieboom, J., Dennis, J. J., de Bont, J. A. M., & Zylstra, G. J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1998-01
- Volume
- 273
- Issue
- 1
- Pages
- 85-91
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2112535900
- Cited by
- 254
- References
- 1
- FWCI
- 8.91
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Kieboom, J., Dennis, J. J., de Bont, J. A. M., & Zylstra, G. J. (1998). Identification and Molecular Characterization of an Efflux Pump Involved in Pseudomonas putida S12 Solvent Tolerance. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.273.1.85
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
