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An APAF-1·Cytochrome c Multimeric Complex Is a Functional Apoptosome That Activates Procaspase-9
Open accessAbstract
We report here the reconstitution of the de novo procaspase-9 activation pathway using highly purified cytochrome c, recombinant APAF-1, and recombinant procaspase-9. APAF-1 binds and hydrolyzes ATP or dATP to ADP or dADP, respectively. The hydrolysis of ATP/dATP and the binding of cytochrome c promote APAF-1 oligomerization, forming a large multimeric APAF-1.cytochrome c complex. Such a complex can be isolated using gel filtration chromatography and is by itself sufficient to recruit and activate procaspase-9. The stoichiometric ratio of procaspase-9 to APAF-1 is approximately 1 to 1 in the complex. Once activated, caspase-9 disassociates from the complex and becomes available to cleave and activate downstream caspases such as caspase-3.
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Metadata
- Authors
- Zou, H., Li, Y., Liu, X., & Wang, X.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1999-04
- Volume
- 274
- Issue
- 17
- Pages
- 11549-11556
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1506642042
- Cited by
- 2,090
- References
- 0
- FWCI
- 45.35
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: An APAF-1·Cytochrome c Multimeric Complex Is a Functional Apoptosome That Activates Procaspase-9. https://app.cochat.ai/papers/10.1074/jbc.274.17.11549
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- Zou, H., Li, Y., Liu, X., & Wang, X. (1999). An APAF-1·Cytochrome c Multimeric Complex Is a Functional Apoptosome That Activates Procaspase-9. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.274.17.11549
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