The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Sustained Mitogen-activated Protein Kinase (MAPK) and Cytoplasmic Phospholipase A2 Activation by Macrophage Migration Inhibitory Factor (MIF)
Open accessAbstract
Macrophage migration inhibitory factor (MIF) is an important pro-inflammatory mediator with the unique ability to counter-regulate the inhibitory effects of glucocorticoids on immune cell activation. MIF is released from cells in response to glucocorticoids, certain pro-inflammatory stimuli, and mitogens and acts to regulate glucocorticoid action on the ensuing inflammatory response. To gain insight into the molecular mechanism of MIF action, we have examined the role of MIF in the proliferation and intracellular signaling events of the well characterized, NIH/3T3 fibroblast cell line. Both endogenously secreted and exogenously added MIFs stimulate the proliferation of NIH/3T3 cells, and this response is associated with the activation of the p44/p42 extracellular signal-regulated (ERK) mitogen-activated protein kinases (MAP). The MIF-induced activation of these kinases was sustained for a period of at least 24 h and was dependent upon protein kinase A activity. We further show that MIF regulates cytosolic phospholipase A2 activity via a protein kinase A and ERK dependent pathway and that the glucocorticoid suppression of cytokine-induced cytoplasmic phospholipase A2 activity and arachidonic acid release can be reversed by the addition of recombinant MIF. These studies indicate that the sustained activation of p44/p42 MAP kinase and subsequent arachidonate release by cytoplasmic phospholipase A2 are important features of the immunoregulatory and intracellular signaling events initiated by MIF and provide the first insight into the mechanisms that underlie the pro-proliferative and inflammatory properties of this mediator.
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Cites
A protein kinase involved in the regulation of inflammatory cytokine biosynthesis
Lee, J. C., Laydon, J. T., McDonnell, P. C., Gallagher, T. F., Kumar, S., Green, D., McNulty, D., Blumenthal, M. J., Keys, J. R., Land vatter, S. W., et al. (1994) · Nature · doi:10.1038/372739a0 · Cited by 3,288
cPLA2 is phosphorylated and activated by MAP kinase
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MIF as a glucocorticoid-induced modulator of cytokine production
Calandra, T., Bernhagen, J., Metz, C. N., Spiegel, L. A., Bacher, M., Donnelly, T., Cerami, A., & Bucala, R. (1995) · Nature · doi:10.1038/377068a0 · Cited by 1,149
Self-citationcAMP Activates MAP Kinase and Elk-1 through a B-Raf- and Rap1-Dependent Pathway
Vossler, M. R., Yao, H., York, R. D., Pan, M.-G., Rim, C. S., & Stork, P. J. S. (1997) · Cell · doi:10.1016/s0092-8674(00)80184-1 · Cited by 1,046
MIF is a pituitary-derived cytokine that potentiates lethal endotoxaemia
Bernhagen, J., Calandra, T., Mitchell, R. A., Martin, S. B., Tracey, K. J., Voelter, W., Manogue, K. R., Cerami, A., & Bucala, R. (1993) · Nature · doi:10.1038/365756a0 · Cited by 999
Self-citationCalandra, T., Bernhagen, J., Mitchell, R. A., & Bucala, R. (1994) · The Journal of experimental medicine · doi:10.1084/jem.179.6.1895 · Cited by 985
Self-citationTraverse, S., Gomez, N., Paterson, H., Marshall, C., & Cohen, P. (1992) · Biochemical Journal · doi:10.1042/bj2880351 · Cited by 874
An essential regulatory role for macrophage migration inhibitory factor in T-cell activation.
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Self-citationDENHARDT, D. T. (1996) · Biochemical Journal · doi:10.1042/bj3180729 · Cited by 495
Cited by
MIF Signal Transduction Initiated by Binding to CD74
Leng, L., Metz, C. N., Fang, Y., Xu, J., Donnelly, S., Baugh, J., Delohery, T., Chen, Y., Mitchell, R. A., & Bucala, R. (2003) · The Journal of Experimental Medicine · doi:10.1084/jem.20030286 · Cited by 1,154
Self-citationMitchell, R. A., Liao, H., Chesney, J., Fingerle-Rowson, G., Baugh, J., David, J., & Bucala, R. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.012511599 · Cited by 622
Self-citationMiller, E. J., Li, J., Leng, L., McDonald, C., Atsumi, T., Bucala, R., & Young, L. H. (2008) · Nature · doi:10.1038/nature06504 · Cited by 429
Self-citationLue, H., Thiele, M., Franz, J., Dahl, E., Speckgens, S., Leng, L., Fingerle-Rowson, G., Bucala, R., Lüscher, B., & Bernhagen, J. (2007) · Oncogene · doi:10.1038/sj.onc.1210318 · Cited by 385
Self-citationNair, A., & Bonneau, R. H. (2006) · Journal of Neuroimmunology · doi:10.1016/j.jneuroim.2005.09.012 · Cited by 392
Metadata
- Authors
- Mitchell, R. A., Metz, C. N., Peng, T., & Bucala, R.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1999-06
- Volume
- 274
- Issue
- 25
- Pages
- 18100-18106
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2049310294
- Cited by
- 448
- References
- 42
- FWCI
- 5.34
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Sustained Mitogen-activated Protein Kinase (MAPK) and Cytoplasmic Phospholipase A2 Activation by Macrophage Migration Inhibitory Factor (MIF). https://app.cochat.ai/papers/10.1074/jbc.274.25.18100
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- Mitchell, R. A., Metz, C. N., Peng, T., & Bucala, R. (1999). Sustained Mitogen-activated Protein Kinase (MAPK) and Cytoplasmic Phospholipase A2 Activation by Macrophage Migration Inhibitory Factor (MIF). Journal of Biological Chemistry. https://doi.org/10.1074/jbc.274.25.18100
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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