The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Characterization of a Novel Sphingosine 1-Phosphate Receptor, Edg-8
Open accessAbstract
Three G protein-coupled receptors (Edg-1, Edg-3, and Edg-5) for the lysolipid phosphoric acid mediator sphingosine 1-phosphate have been described by molecular cloning. Using a similar sequence that we found in the expressed sequence tag data base, we cloned and characterized of a fourth, high affinity, rat brain sphingosine 1-phosphate receptor, Edg-8. When HEK293T cells were co-transfected with Edg-8 and G protein DNAs, prepared membranes showed sphingosine 1- phosphate-dependent increases in [(35)S]guanosine 5'-(3-O-thio)triphosphate binding with an EC(50) of 90 nm. In a rat hepatoma Rh7777 cell line that exhibits modest endogenous responses to sphingosine 1-phosphate, this lipid mediator inhibited forskolin-driven rises in cAMP by greater than 90% when the cells were transfected with Edg-8 DNA (IC(50) 0.7 nm). This response is blocked fully by prior treatment of cultures with pertussis toxin, thus implicating signaling through G(i/o)alpha proteins. Furthermore, Xenopus oocytes exhibit a calcium response to sphingosine 1-phosphate after injection of Edg-8 mRNA, but only when oocytes are co-injected with chimeric G(q/i)alpha protein mRNA. Membranes from HEK293T and Rh7777 cell cultures expressing Edg-8 exhibited high affinity (K(D) = 2 nm) binding for radiolabeled sphingosine 1-phosphate. Rat Edg-8 RNA is expressed in spleen and throughout adult rat brain where in situ hybridization revealed it to be associated with white matter. Together our data demonstrate that Edg-8 is a high affinity sphingosine 1-phosphate receptor that couples to G(i/o)alpha proteins and is expressed predominantly by oligodendrocytes and/or fibrous astrocytes in the rat brain.
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Self-citationSphingosine-1-Phosphate as a Ligand for the G Protein-Coupled Receptor EDG-1
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Lysophospholipid Receptors: Signaling and Biology
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Self-citationNatural killer cell trafficking in vivo requires a dedicated sphingosine 1-phosphate receptor
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Metadata
- Authors
- Im, D.-S., Heise, C. E., Ancellin, N., O'Dowd, B. F., Shei, G.-J., Heavens, R. P., Rigby, M. R., Hla, T., Mandala, S., McAllister, G., George, S. R., & Lynch, K. R.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2000-05
- Volume
- 275
- Issue
- 19
- Pages
- 14281-14286
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2155487600
- Cited by
- 320
- References
- 25
- FWCI
- 11.94
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Im, D.-S., Heise, C. E., Ancellin, N., O'Dowd, B. F., Shei, G.-J., Heavens, R. P., Rigby, M. R., Hla, T., Mandala, S., McAllister, G., George, S. R., & Lynch, K. R. (2000). Characterization of a Novel Sphingosine 1-Phosphate Receptor, Edg-8. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.275.19.14281
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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