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Mechanism and Cellular Applications of a Green Fluorescent Protein-based Halide Sensor

Jayaraman, Sujatha, Haggie, Peter, Wachter, Rebekka M., Remington, S.James, Verkman, A.S.·2000·Journal of Biological Chemistry
DOI 10.1074/jbc.275.9.6047
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FIG. 1 Sensitivity of YFP-H148Q spectral properties to Cl 2 . A , dependence of YFP-H148Q fluorescence on pH at indicated [Cl 2 ]. B , dependence of relative YFP-H148Q fluorescence on [Cl 2 ] or [I 2 ] at pH 7.5. C , influence of Cl 2 on YFP-H148Q absorption spectrum at pH 6.4. D , influence of fluoride on YFP-H148Q CD spectrum.
FIG. 1 Sensitivity of YFP-H148Q spectral properties to Cl 2 . A , dependence of YFP-H148Q fluorescence on pH at indicated [Cl 2 ]. B , dependence of relative YFP-H148Q fluorescence on [Cl 2 ] or [I 2 ] at pH 7.5. C , influence of Cl 2 on YFP-H148Q absorption spectrum at pH 6.4. D , influence of fluoride on YFP-H148Q CD spectrum.CC-BY · From the PDF
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FIG. 3 YFP-H148Q expression in transfected Swiss 3T3 fibroblasts. A , fluorescence confocal micrograph showing expression in cytoplasm and nucleus. B , dependence of intracellular YFP-H148Q fluorescence on pH. Fluorescence pH titration (at 0 Cl 2 ) in Swiss 3T3 fibroblasts expressing YFP-H148Q and CFTR. Cells were perfused with high K 1 buffer containing ionophores at indicated pH (see 'Materials and Methods'). C , fluorescence Cl 2 titration (at pH 7.4) using high K 1 buffer containing ionophores and indicated [Cl 2 ] (see 'Materials and Methods').
FIG. 3 YFP-H148Q expression in transfected Swiss 3T3 fibroblasts. A , fluorescence confocal micrograph showing expression in cytoplasm and nucleus. B , dependence of intracellular YFP-H148Q fluorescence on pH. Fluorescence pH titration (at 0 Cl 2 ) in Swiss 3T3 fibroblasts expressing YFP-H148Q and CFTR. Cells were perfused with high K 1 buffer containing ionophores at indicated pH (see 'Materials and Methods'). C , fluorescence Cl 2 titration (at pH 7.4) using high K 1 buffer containing ionophores and indicated [Cl 2 ] (see 'Materials and Methods').CC-BY · From the PDF
FIG. 4 CFTR-mediated Cl 2 transport detected by changes in intracellular YFP-H148Q fluorescence. A , CFTR-expressing cells were perfused with PBS containing forskolin to activate CFTR. Where indicated Cl 2 was replaced with gluconate ( top ), NO3 2 ( middle ), or I 2 ( bottom ). B , forskolin-stimulated Cl 2 /I 2 transport. Cells ( top , CFTRexpressing; bottom , control) were initially perfused with a phosphate buffer containing 100 mM I 2 followed by perfusion with PBS and then addition of 20 m M forskolin as indicated.
FIG. 4 CFTR-mediated Cl 2 transport detected by changes in intracellular YFP-H148Q fluorescence. A , CFTR-expressing cells were perfused with PBS containing forskolin to activate CFTR. Where indicated Cl 2 was replaced with gluconate ( top ), NO3 2 ( middle ), or I 2 ( bottom ). B , forskolin-stimulated Cl 2 /I 2 transport. Cells ( top , CFTRexpressing; bottom , control) were initially perfused with a phosphate buffer containing 100 mM I 2 followed by perfusion with PBS and then addition of 20 m M forskolin as indicated.CC-BY · From the PDF

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Research works printed but not linked: 29

  1. Mansoura, 1999, Hum. Gene Ther., 10, 861, 10.1089/10430349950018274 · DOI
  2. Verkman, 1990, Am. J. Physiol., 259, C375, 10.1152/ajpcell.1990.259.3.C375 · DOI
  3. Biwersi, 1991, Biochemistry, 30, 7879, 10.1021/bi00246a001 · DOI
  4. Jayaraman, 1999, Am. J. Physiol., 276, C747, 10.1152/ajpcell.1999.276.3.C747 · DOI
  5. Biwersi, 1994, Anal. Biochem., 219, 139, 10.1006/abio.1994.1242 · DOI
  6. Jayaraman, 1999, Am. J. Physiol., 277, C1008, 10.1152/ajpcell.1999.277.5.C1008 · DOI
  7. De Giorgi, 1996, Gene (Amst.), 173, 113, 10.1016/0378-1119(95)00687-7 · DOI
  8. De Giorgi, 1999, Methods Cell Biol., 58, 75, 10.1016/S0091-679X(08)61949-4 · DOI
  9. Rizzuto, 1996, Curr. Biol., 6, 183, 10.1016/S0960-9822(02)00451-7 · DOI
  10. Rizzuto, 1995, Curr. Biol., 5, 635, 10.1016/S0960-9822(95)00128-X · DOI
  11. Cole, 1996, Science, 273, 797, 10.1126/science.273.5276.797 · DOI
  12. Kneen, 1998, Biophys. J., 74, 1591, 10.1016/S0006-3495(98)77870-1 · DOI
  13. Llopis, 1998, Proc. Natl. Acad. Sci. U. S. A., 95, 6803, 10.1073/pnas.95.12.6803 · DOI
  14. Miesenbock, 1998, Nature, 394, 192, 10.1038/28190 · DOI
  15. Miyawaki, 1997, Nature, 388, 882, 10.1038/42264 · DOI
  16. Romoser, 1997, J. Biol. Chem., 272, 13270, 10.1074/jbc.272.20.13270 · DOI
  17. Siegel, 1997, Neuron, 19, 735, 10.1016/S0896-6273(00)80955-1 · DOI
  18. Heim, 1995, Nature, 373, 663, 10.1038/373663b0 · DOI
  19. Heim, 1996, Curr. Biol., 6, 1178, 10.1016/S0960-9822(02)00450-5 · DOI
  20. Ormo, 1996, Science, 273, 1392, 10.1126/science.273.5280.1392 · DOI
  21. Elsliger, 1999, Biochemistry, 38, 5296, 10.1021/bi9902182 · DOI
  22. Wachter, 1998, Structure (Lond.), 6, 1267, 10.1016/S0969-2126(98)00127-0 · DOI
  23. Wachter, 1999, Curr. Biol., 9, R628, 10.1016/S0960-9822(99)80408-4 · DOI
  24. Fushimi, 1991, J. Cell Biol., 112, 719, 10.1083/jcb.112.4.719 · DOI
  25. Partikian, 1998, J. Cell Biol., 140, 821, 10.1083/jcb.140.4.821 · DOI
  26. Halm, 1992, J. Gen. Physiol., 99, 339, 10.1085/jgp.99.3.339 · DOI
  27. Swaminathan, 1997, Biophys. J., 72, 1900, 10.1016/S0006-3495(97)78835-0 · DOI
  28. Periasamy, 1996, Photochem. Photobiol., 63, 265, 10.1111/j.1751-1097.1996.tb03023.x · DOI
  29. Dawson, 1999, Physiol. Rev., 79, S47, 10.1152/physrev.1999.79.1.S47 · DOI

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Metadata

Authors
Jayaraman, S., Haggie, P., Wachter, R. M., Remington, S. J., & Verkman, A. S.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2000-03
Volume
275
Issue
9
Pages
6047-6050
ISSN
0021-9258
DOI
10.1074/jbc.275.9.6047
Cited by
264
References
0
Language
en
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CoChat. (2026). Citation check: Mechanism and Cellular Applications of a Green Fluorescent Protein-based Halide Sensor. https://app.cochat.ai/papers/10.1074/jbc.275.9.6047
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Jayaraman, S., Haggie, P., Wachter, R. M., Remington, S. J., & Verkman, A. S. (2000). Mechanism and Cellular Applications of a Green Fluorescent Protein-based Halide Sensor. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.275.9.6047