In Situ Determination of Manganese(II) Speciation in Deinococcus radiodurans by High Magnetic Field EPR
Open accessAbstract
High magnetic field high frequency electron paramagnetic resonance techniques were used to measure in situ Mn(II) speciation in Deinococcus radiodurans , a radiation-resistant bacteria capable of accumulating high concentrations of Mn(II). It was possible to identify and quantify the evolution of Mn(II) species in intact cells at various stages of growth. Aside from water, 95-GHz high field electron nuclear double resonance showed that the Mn(II) ions are bound to histidines and phosphate groups, mostly from fructose-1,6-bisphosphate but also inorganic phosphates and nucleotides. During stationary growth phase, 285-GHz continuous wave EPR measurements showed that histidine is the most common ligand to Mn(II) and that significant amounts of cellular Mn(II) in D. radiodurans are bound to peptides and proteins. As much as 40% of the total Mn(II) was in manganese superoxide dismutase, and it is this protein and not smaller manganese complexes, as has been suggested recently, that is probably the primary defense against superoxide. Background: Mn(II) plays a role in cellular defense against oxidative stress. Results: The Mn(II) speciation in D. radiodurans cells has been determined in situ . Conclusion: Manganese superoxide dismutase is the predominant Mn(II) species and is likely to be more important than small Mn(II) complexes for regulating superoxide. Significance: This work establishes a method for studying Mn(II) speciation and homeostasis in intact cells.
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Manganese accumulates bound to inorganic phosphate and phosphorylated metabolites, small peptides, and nucleosides.
“Manganese accumulates bound to inorganic phosphate and phosphorylated metabolites (e.g., fructose-bisphosphate), small peptides, and nucleosides.106,116”
From Metals in Motion: Understanding Labile Metal Pools in Bacteria (2025) · cites this paper as [106]
The Results and Discussion (page not provided) identify Mn(II) associated with orthophosphate and fructose 1,6-bisphosphate, and report a histidine-ligated metallopeptide/protein in stationary-phase cells. They identify binding to nucleotides or DNA, not nucleosides, and do not establish that the metallopeptide/protein is a small peptide. (LLM, conf=0.86)
Experimental — accuracy not yet measuredIn D. radiodurans, MnSOD can account for 40% of intracellular manganese.
“Similarly, in the highly radiation resistant Gram-negative bacterium Deinococcus radiodurans, MnSOD can account for 40% of intracellular manganese.106”
From Metals in Motion: Understanding Labile Metal Pools in Bacteria (2025) · cites this paper as [106]
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Manganese homeostasis and utilization in pathogenic bacteria
Juttukonda, L. J., & Skaar, E. P. (2015) · Molecular Microbiology · doi:10.1111/mmi.13034 · Cited by 121 · see citing works
Metals in Motion: Understanding Labile Metal Pools in Bacteria
Helmann, J. D. (2025) · Biochemistry · doi:10.1021/acs.biochem.4c00726 · Cited by 49 · see citing works
- Authors
- Tabares, L. C., & Un, S.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Tabares, Leandro C.
- Un, Sun
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2013-02
- Volume
- 288
- Issue
- 7
- Pages
- 5050-5055
- ISSN
- 0021-9258 (ISSN-L)
- PMC
- PMC3576107
- OpenAlex
- W1972494716
- Cited by
- 47
- References
- 1
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 1 · 2019: 5 · 2018: 1 · 2017: 4 · 2016: 4 · 2015: 10 · 2014: 5 · 2013: 3
- FWCI
- 4.44
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Electron Spin Resonance Studies
- Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Catalyzed Oxygenation Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- Deinococcus radiodurans, manganese superoxide dismutase, manganese homeostasis, fructose-1,6-bisphosphate, oxidative stress, radioresistance (biology)
- MeSH terms
- DNA, Electron Spin Resonance Spectroscopy, Homeostasis, Ions, Ligands, Manganese, Magnetic Resonance Spectroscopy, Nucleotides, Phosphates, Protein Binding, Superoxides, Superoxide Dismutase, Time Factors, Oxidative Stress, Deinococcus
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