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Dual Targeting of Spinach Protoporphyrinogen Oxidase II to Mitochondria and Chloroplasts by Alternative Use of Two In-frame Initiation Codons
Open accessAbstract
Protoporphyrinogen oxidase (Protox) is the final enzyme in the common pathway of chlorophyll and heme biosynthesis. Two Protox isoenzymes have been described in tobacco, a plastidic and a mitochondrial form. We isolated and sequenced spinach Protox cDNA, which encodes a homolog of tobacco mitochondrial Protox (Protox II). Alignment of the deduced amino acid sequence between Protox II and other tobacco mitochondrial Protox homologs revealed a 26-amino acid N-terminal extension unique to the spinach enzyme. Immunoblot analysis of spinach leaf extract detected two proteins with apparent molecular masses of 57 and 55 kDa in chloroplasts and mitochondria, respectively. In vitro translation experiments indicated that two translation products (59 and 55 kDa) are produced from Protox II mRNA, using two in-frame initiation codons. Transport experiments using green fluorescent protein-fused Protox II suggested that the larger and smaller translation products (Protox IIL and IIS) target exclusively to chloroplasts and mitochondria, respectively.
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Citations
Cited by
Tetrapyrrole Biosynthesis in Higher Plants
Tanaka, R., & Tanaka, A. (2007) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.57.032905.105448 · Cited by 818
Tanaka, A., & Tanaka, R. (2006) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2006.03.011 · Cited by 409
Recent advances in chlorophyll biosynthesis and breakdown in higher plants
Eckhardt, U., Grimm, B., & H�rtensteiner, S. (2004) · Plant Molecular Biology · doi:10.1007/s11103-004-2331-3 · Cited by 388
The cell biology of tetrapyrroles: a life and death struggle
Mochizuki, N., Tanaka, R., Grimm, B., Masuda, T., Moulin, M., Smith, A. G., Tanaka, A., & Terry, M. J. (2010) · Trends in Plant Science · doi:10.1016/j.tplants.2010.05.012 · Cited by 320
The biochemistry of heme biosynthesis
Heinemann, I. U., Jahn, M., & Jahn, D. (2008) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2008.02.015 · Cited by 311
Metadata
- Authors
- Watanabe, N., Che, F.-S., Iwano, M., Takayama, S., Yoshida, S., & Isogai, A.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2001-01
- Volume
- 276
- Issue
- 23
- Pages
- 20474-20481
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2018363274
- Cited by
- 170
- References
- 0
- FWCI
- 3.26
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Watanabe, N., Che, F.-S., Iwano, M., Takayama, S., Yoshida, S., & Isogai, A. (2001). Dual Targeting of Spinach Protoporphyrinogen Oxidase II to Mitochondria and Chloroplasts by Alternative Use of Two In-frame Initiation Codons. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m101140200
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