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Yeast Sco1, a Protein Essential for Cytochrome cOxidase Function Is a Cu(I)-binding Protein
Open accessAbstract
Sco1 is a conserved essential protein, which has been implicated in the delivery of copper to cytochrome c oxidase, the last enzyme of the electron transport chain. In this study, we show for the first time that the purified C-terminal domain of yeast Sco1 binds one Cu(I)/monomer. X-ray absorption spectroscopy suggests that the Cu(I) is ligated via three ligands, and we show that two cysteines, present in a conserved motif CXXXC, and a conserved histidine are involved in Cu(I) ligation. The mutation of any one of the conserved residues in Sco1 expressed in yeast abrogates the function of Sco1 resulting in a non-functional cytochrome c oxidase complex. Thus, the function of Sco1 correlates with Cu(I) binding. Data obtained from size-exclusion chromatography experiments with mitochondrial lysates suggest that full-length Sco1 may be oligomeric in vivo.
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Citations
Cited by
Molecular Analysis of the Copper-Transporting Efflux System CusCFBA of Escherichia coli
Franke, S., Grass, G., Rensing, C., & Nies, D. H. (2003) · Journal of Bacteriology · doi:10.1128/jb.185.13.3804-3812.2003 · Cited by 538
Stern, B. R., Solioz, M., Krewski, D., Aggett, P., Aw, T.-C., Baker, S., Crump, K., Dourson, M., Haber, L., Hertzberg, R., et al. (2007) · Journal of Toxicology and Environmental Health, Part B · doi:10.1080/10937400600755911 · Cited by 373
Copper trafficking to the mitochondrion and assembly of copper metalloenzymes
Cobine, P. A., Pierrel, F., & Winge, D. R. (2006) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2006.03.002 · Cited by 317
Self-citationMitochondrial copper metabolism and delivery to cytochrome c oxidase
Horn, D., & Barrientos, A. (2008) · IUBMB Life · doi:10.1002/iub.50 · Cited by 283
Metal Acquisition and Availability in the Mitochondria
Atkinson, A., & Winge, D. R. (2009) · Chemical Reviews · doi:10.1021/cr900006y · Cited by 146
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Metadata
- Authors
- Nittis, T., George, G. N., & Winge, D. R.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2001-11
- Volume
- 276
- Issue
- 45
- Pages
- 42520-42526
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2087813537
- Cited by
- 183
- References
- 0
- FWCI
- 4.67
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Nittis, T., George, G. N., & Winge, D. R. (2001). Yeast Sco1, a Protein Essential for Cytochrome cOxidase Function Is a Cu(I)-binding Protein. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m107077200
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