The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
EWI-2 Is a Major CD9 and CD81 Partner and Member of a Novel Ig Protein Subfamily
Open accessAbstract
A novel Ig superfamily protein, EWI-2, was co-purified with tetraspanin protein CD81 under relatively stringent Brij 96 detergent conditions and identified by mass spectrometric protein sequencing. EWI-2 associated specifically with CD9 and CD81 but not with other tetraspanins or with integrins. Immunodepletion experiments indicated that EWI-2-CD9/CD81 interactions are highly stoichiometric, with approximately 70% of CD9 and CD81 associated with EWI-2 in an embryonic kidney cell line. The EWI-2 molecule was covalently cross-linked (in separate complexes) to both CD81 and CD9, suggesting that association is direct. EWI-2 is part of a novel Ig subfamily that includes EWI-F (F2alpha receptor regulatory protein (FPRP), CD9P-1), EWI-3 (IgSF3), and EWI-101 (CD101). All four members of this Ig subfamily contain a Glu-Trp-Ile (EWI) motif not seen in other Ig proteins. As shown previously, the EWI-F molecule likewise forms highly proximal, specific, and stoichiometric complexes with CD9 and CD81. Human and murine EWI-2 protein sequences are 91% identical, and transcripts in the two species are expressed in virtually every tissue tested. Thus, EWI-2 potentially contributes to a variety of CD9 and CD81 functions seen in different cell and tissue types.
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Kibar, Z., Vogan, K. J., Groulx, N., Justice, M. J., Underhill, D. A., & Gros, P. (2001) · Nature Genetics · doi:10.1038/90081 · Cited by 504
CD81 Regulates Neuron-Induced Astrocyte Cell-Cycle Exit
Kelić, S., Levy, S., Suarez, C., & Weinstein, D. E. (2001) · Molecular and Cellular Neuroscience · doi:10.1006/mcne.2000.0955 · Cited by 60
Cited by
Tetraspanin functions and associated microdomains
Hemler, M. E. (2005) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1736 · Cited by 1,355
Self-citationPerez-Hernandez, D., Gutiérrez-Vázquez, C., Jorge, I., López-Martín, S., Ursa, A., Sánchez-Madrid, F., Vázquez, J., & Yáñez-Mó, M. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.445304 · Cited by 532
Myelin Proteomics: Molecular Anatomy of an Insulating Sheath
Jahn, O., Tenzer, S., & Werner, H. B. (2009) · Molecular Neurobiology · doi:10.1007/s12035-009-8071-2 · Cited by 329
Little, K. D., Hemler, M. E., & Stipp, C. S. (2004) · Molecular Biology of the Cell · doi:10.1091/mbc.e03-12-0886 · Cited by 211
Self-citationJouannet, S., Saint-Pol, J., Fernandez, L., Nguyen, V., Charrin, S., Boucheix, C., Brou, C., Milhiet, P.-E., & Rubinstein, E. (2016) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-015-2111-z · Cited by 138
Metadata
- Authors
- Stipp, C. S., Kolesnikova, T. V., & Hemler, M. E.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2001-11
- Volume
- 276
- Issue
- 44
- Pages
- 40545-40554
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2042397395
- Cited by
- 235
- References
- 2
- FWCI
- 4.50
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Stipp, C. S., Kolesnikova, T. V., & Hemler, M. E. (2001). EWI-2 Is a Major CD9 and CD81 Partner and Member of a Novel Ig Protein Subfamily. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m107338200
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