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Cited by 286

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Structure Determination and Improved Model of Plant Photosystem I

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Amunts, Alexey, Toporik, Hila, Borovikova, Anna, Nelson, Nathan·2010·Journal of Biological Chemistry

Abstract

Photosystem I functions as a sunlight energy converter, catalyzing one of the initial steps in driving oxygenic photosynthesis in cyanobacteria, algae, and higher plants. Functionally, Photosystem I captures sunlight and transfers the excitation energy through an intricate and precisely organized antenna system, consisting of a pigment network, to the center of the molecule, where it is used in the transmembrane electron transfer reaction. Our current understanding of the sophisticated mechanisms underlying these processes has profited greatly from elucidation of the crystal structures of the Photosystem I complex. In this report, we describe the developments that ultimately led to enhanced structural information of plant Photosystem I. In addition, we report an improved crystallographic model at 3.3-A resolution, which allows analysis of the structure in more detail. An improved electron density map yielded identification and tracing of subunit PsaK. The location of an additional ten beta-carotenes as well as five chlorophylls and several loop regions, which were previously uninterpretable, are now modeled. This represents the most complete plant Photosystem I structure obtained thus far, revealing the locations of and interactions among 17 protein subunits and 193 non-covalently bound photochemical cofactors. Using the new crystal structure, we examine the network of contacts among the protein subunits from the structural perspective, which provide the basis for elucidating the functional organization of the complex.

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Metadata

Authors
Amunts, A., Toporik, H., Borovikova, A., & Nelson, N.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2010-01
Volume
285
Issue
5
Pages
3478-3486
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2145597436
Cited by
286
References
50
FWCI
7.68
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Structure Determination and Improved Model of Plant Photosystem I. https://app.cochat.ai/papers/10.1074/jbc.m109.072645
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Amunts, A., Toporik, H., Borovikova, A., & Nelson, N. (2010). Structure Determination and Improved Model of Plant Photosystem I. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m109.072645

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.