Keratinocyte-derived Laminin-332 Protein Promotes Melanin Synthesis via Regulation of Tyrosine Uptake
Open accessAbstract
Melanocytes, which produce the pigment melanin, are known to be closely regulated by neighboring keratinocytes. However, how keratinocytes regulate melanin production is unclear. Here we report that melanin production in melanoma cells (B16F10 and MNT-1) was increased markedly on a keratinocyte-derived extracellular matrix compared with a melanoma cell-derived extracellular matrix. siRNA-mediated reduction of keratinocyte-derived laminin-332 expression decreased melanin synthesis in melanoma cells, and laminin-332, but not fibronectin, enhanced melanin content and α-melanocyte-stimulating hormone-regulated melanin production in melanoma cells. Similar effects were observed in human melanocytes. Interestingly, however, laminin-332 did not affect the expression or activity of tyrosinase. Instead, laminin-332 promoted the uptake of extracellular tyrosine and, subsequently, increased intracellular levels of tyrosine in both melanocytes and melanoma cells. Taken together, these data strongly suggest that keratinocyte-derived laminin-332 contributes to melanin production by regulating tyrosine uptake.
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Ando, H., Niki, Y., Ito, M., Akiyama, K., Matsui, M. S., Yarosh, D. B., & Ichihashi, M. (2012) · Journal of Investigative Dermatology · doi:10.1038/jid.2011.413 · Cited by 258 · see citing works
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Duval, C., Cohen, C., Chagnoleau, C., Flouret, V., Bourreau, E., & Bernerd, F. (2014) · PLoS ONE · doi:10.1371/journal.pone.0114182 · Cited by 114 · see citing works
Zhao, G., Lyu, J., Veniaminova, N. A., Zarnowski, R., Mattos, E., Johnson, C. J., Quintanilla, D., Hautau, H., Hold, L. A., Xu, B., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-60876-1 · Cited by 32 · see citing works
“Human hair follicles are embedded in the dermis, which contains a complex mixture of ECM proteins, including laminin 35 , which are essential for the structural integrity and function of the skin.”
Zhao, G., Lyu, J., Veniaminova, N. A., Zarnowski, R., Mattos, E., Johnson, C. J., Quintanilla, D., Hautau, H., Hold, L. A., Xu, B., et al. (2025) · doi:10.1101/2025.02.13.637978 · Cited by 2 · see citing works
- Authors
- Chung, H., Jung, H., Lee, J.-H., Oh, H. Y., Kim, O. B., Han, I.-O., & Oh, E.-S.
- Affiliations
- Ewha Womans University (KR)
- Inha University (KR)
- Corresponding author
- Oh, Eok-Soo
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2014-08
- Volume
- 289
- Issue
- 31
- Pages
- 21751-21759
- ISSN
- 0021-9258 (ISSN-L)
- PMC
- PMC4118133
- OpenAlex
- W2050840167
- Cited by
- 30
- References
- 1
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 2 · 2023: 3 · 2022: 1 · 2021: 2 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 5 · 2015: 3 · 2014: 1
- FWCI
- 1.99
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Research Foundation (2012R1A5A1048236)
- National Research Foundation of Korea (2012R1A5A1048236)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Melanoma and MAPK Pathways
- Health Sciences › Medicine › Oncology › Cutaneous Melanoma Detection and Management
- Keywords
- laminin-5, melanogenesis, keratinocytes, melanocytes, melanoma cells, tyrosine uptake, extracellular matrix, alpha-melanocyte-stimulating hormone, tyrosinase, fibronectin
- MeSH terms
- Kalinin, alpha-MSH, Animals, Base Sequence, Humans, Melanins, Melanoma, Experimental, Tyrosine, Keratinocytes, Cell Adhesion Molecules, RNA, Small Interfering, Cell Line, Tumor, Mice
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