Checkpoint arrest signaling in response to UV damage is independent of nucleotide excision repair in Saccharomyces cerevisiae
Open accessAbstract
The recognition of DNA double-stranded breaks or single-stranded DNA gaps as a precondition for cell cycle checkpoint arrest has been well established. However, how bulky base damage such as UV-induced pyrimidine dimers elicits a checkpoint response has remained elusive. Nucleotide excision repair represents the main pathway for UV dimer removal that results in strand interruptions. However, we demonstrate here that Rad53p hyperphosphorylation, an early event of checkpoint signaling in Saccharomyces cerevisiae, is independent of nucleotide excision repair (NER), even if replication as a source of secondary DNA damage is excluded. Thus, our data hint at primary base damage or at UV damage (primary or secondary) that does not need to be processed by NER as the relevant substrate of damage-sensing checkpoint proteins.
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In yeast, UV-induced DNA damage causes checkpoint activation without NER lesion processing.
“In yeast, UV-induced DNA damage results in checkpoint activation independent of NER lesion processing [33, 34].”
From NER initiation factors, DDB2 and XPC, regulate UV radiation response by recruiting ATR and ATM kinases to DNA damage sites (2013) · cites this paper as [34]
A NER-independent mechanism for activating checkpoint kinases seems to exist in non-cycling cells; NER-deficient yeast blocked in G1 and UV irradiated show delayed Mec1-dependent pathway activation.
“Interestingly, a NER-independent mechanism for activating checkpoint kinases seems to exist in non cycling cells. If NER-deficient yeast cells are blocked in the G1 phase of the cell cycle, UV irradiated and held in non dividing conditions indefinitely, a delayed activation of the Mec1-dependent pathway has been reported [95,96].”
From Mind the gap: keeping UV lesions in check (2011) · cites this paper as [95]
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References 51 · Cited by 24
Research works printed but not linked: 9
- Jackson, S. P. (2001) Biochem. Soc. Trans. 29, 655-661
- Lindsey-Boltz, L. A., Bermudez, V. P., Hurwitz, J., and Sancar, A. (2001) Proc. Natl. Acad. Sci. U. S. A. 98, 11236 -11241
- Huang, L.-C., Clarkin, K. C., and Wahl, G. M. (1996) Proc. Natl. Acad. Sci.
- Friedberg, E. C., Walker, G. C., and Siede, W. (1995) DNA Repair and Mu- tagenesis, American Society of Microbiology Press, Washington, D. C.
- Siede, W., Friedberg, A. S., and Friedberg, E. C. (1993) Proc. Natl. Acad. Sci.
- Wright, J. A., Keegan, K. S., Herendeen, D. R., Bentley, N. J., Carr, A. M., Hoekstra, M. F., and Concannon, P. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 7445-7450
- Cohen-Fix, O., and Koshland, D. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 14361-14366
- Siede, W. (1998) in DNA Damage and Repair, DNA Repair in Prokaryotes and Lower Eukaryotes (Nickoloff, J. A., and Hoekstra, M. F., eds), Vol. 1, pp. 307-333, Humana Press, Totowa, NJ 45.
- U ¨nsal-Kac ¸maz, K., Makhov, A. M., Griffith, J. D., and Sancar, A. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 6673-6678
- Authors
- Zhang, H., Taylor, J., & Siede, W.
- Affiliations
- Emory University (US)
- Corresponding author
- Siede, Wolfram
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2003-03
- Volume
- 278
- Issue
- 11
- Pages
- 9382-9387
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2006536596
- Cited by
- 24
- References
- 51
- Citations by year
- 2025: 1 · 2014: 1 · 2013: 1
- FWCI
- 1.49
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Cancer Institute (CA87381, R01CA087381)
- National Institute of Environmental Health Sciences (ES01163)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- DNA damage checkpoint, nucleotide excision repair, checkpoint signaling, Saccharomyces cerevisiae
- MeSH terms
- Cell Cycle, Cell Separation, DNA Damage, DNA Repair, Dose-Response Relationship, Radiation, Flow Cytometry, Mitosis, Phosphorylation, Saccharomyces cerevisiae, Temperature, Time Factors, Ultraviolet Rays, Immunoblotting, Signal Transduction, Nocodazole, G1 Phase, Protein Serine-Threonine Kinases, Cell Cycle Proteins, Dimerization, Saccharomyces cerevisiae Proteins, Checkpoint Kinase 2
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