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A Proteomic Study of SUMO-2 Target Proteins
Open accessAbstract
The SUMO family in vertebrates includes at least three distinct proteins (SUMO-1, -2, and -3) that are added as post-translational modifications to target proteins. A considerable number of SUMO-1 target proteins have been identified, but little is known about SUMO-2. A stable HeLa cell line expressing His6-tagged SUMO-2 was established and used to label and purify novel endogenous SUMO-2 target proteins. Tagged forms of SUMO-2 were functional and localized predominantly in the nucleus. His6-tagged SUMO-2 conjugates were affinity-purified from nuclear fractions and identified by mass spectrometry. Eight novel potential SUMO-2 target proteins were identified by at least two peptides. Three of these proteins, SART1, heterogeneous nuclear ribonucleoprotein (RNP) M, and the U5 small nuclear RNP 200-kDa helicase, play a role in RNA metabolism. SART1 and heterogeneous nuclear RNP M were both shown to be genuine SUMO targets, confirming the validity of the approach.
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Cited by
Small Ubiquitin-like Modifier (SUMO) Modification of Natively Unfolded Proteins Tau and α-Synuclein
Dorval, V., & Fraser, P. E. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m510127200 · Cited by 319
Saracco, S. A., Miller, M. J., Kurepa, J., & Vierstra, R. D. (2007) · Plant Physiology · doi:10.1104/pp.107.102285 · Cited by 287
Systematic study of protein sumoylation: Development of a site‐specific predictor of SUMOsp 2.0
Ren, J., Gao, X., Jin, C., Zhu, M., Wang, X., Shaw, A., Wen, L., Yao, X., & Xue, Y. (2009) · PROTEOMICS · doi:10.1002/pmic.200800646 · Cited by 262
Weighing in on ubiquitin: the expanding role of mass-spectrometry-based proteomics
Kirkpatrick, D. S., Denison, C., & Gygi, S. P. (2005) · Nature Cell Biology · doi:10.1038/ncb0805-750 · Cited by 209
Metadata
- Authors
- Vertegaal, A. C. O., Ogg, S. C., Jaffray, E., Rodriguez, M. S., Hay, R. T., Andersen, J. S., Mann, M., & Lamond, A. I.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2004-08
- Volume
- 279
- Issue
- 32
- Pages
- 33791-33798
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2118895416
- Cited by
- 231
- References
- 0
- FWCI
- 5.47
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Vertegaal, A. C. O., Ogg, S. C., Jaffray, E., Rodriguez, M. S., Hay, R. T., Andersen, J. S., Mann, M., & Lamond, A. I. (2004). A Proteomic Study of SUMO-2 Target Proteins. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m404201200
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