The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
PCNA Is a Cofactor for Cdt1 Degradation by CUL4/DDB1-mediated N-terminal Ubiquitination
Open accessAbstract
Cdt1, a protein essential in G1 for licensing of origins for DNA replication, is inhibited in S-phase, both by binding to geminin and degradation by proteasomes. Cdt1 is also degraded after DNA damage to stop licensing of new origins until after DNA repair. Phosphorylation of Cdt1 by cyclin-dependent kinases promotes its binding to SCF-Skp2 E3 ubiquitin ligase, but the Cdk2/Skp2-mediated pathway is not essential for the degradation of Cdt1. Here we show that the N terminus of Cdt1 contains a second degradation signal that is active after DNA damage and in S-phase and is dependent on the interaction of Cdt1 with proliferating cell nuclear antigen (PCNA) through a PCNA binding motif. The degradation involves N-terminal ubiquitination and requires Cul4 and Ddb1 proteins, components of an E3 ubiquitin ligase implicated in protein degradation after DNA damage. Therefore PCNA, the matchmaker for many proteins involved in DNA and chromatin metabolism, also serves to promote the targeted degradation of associated proteins in S-phase or after DNA damage.
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Cites
In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector
Naldini, L., Blömer, U., Gallay, P., Ory, D., Mulligan, R., Gage, F. H., Verma, I. M., & Trono, D. (1996) · Science · doi:10.1126/science.272.5259.263 · Cited by 4,903
Separate domains of p21 involved in the inhibition of Cdk kinase and PCNA
Chen, J., Jackson, P. K., Kirschner, M. W., & Dutta, A. (1995) · Nature · doi:10.1038/374386a0 · Cited by 548
Self-citation
Cited by
Jin, J., Arias, E. E., Chen, J., Harper, J. W., & Walter, J. C. (2006) · Molecular Cell · doi:10.1016/j.molcel.2006.08.010 · Cited by 632
Self-citationRegulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC
Katayama, T., Ozaki, S., Keyamura, K., & Fujimitsu, K. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2314 · Cited by 301
The CRL4Cdt2 ubiquitin ligase targets the degradation of p21Cip1 to control replication licensing
Kim, Y., Starostina, N. G., & Kipreos, E. T. (2008) · Genes & Development · doi:10.1101/gad.1703708 · Cited by 240
Lim, S., Khoo, R., Peh, K. M., Teo, J., Chang, S. C., Ng, S., Beilhartz, G. L., Melnyk, R. A., Johannes, C. W., Brown, C. J., et al. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1920251117 · Cited by 170
HIV-1 Vpr activates the G2 checkpoint through manipulation of the ubiquitin proteasome system
DeHart, J. L., Zimmerman, E. S., Ardon, O., Monteiro-Filho, C. M., Argañaraz, E. R., & Planelles, V. (2007) · Virology Journal · doi:10.1186/1743-422x-4-57 · Cited by 152
Metadata
- Authors
- Senga, T., Sivaprasad, U., Zhu, W., Park, J. H., Arias, E. E., Walter, J. C., & Dutta, A.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2006-03
- Volume
- 281
- Issue
- 10
- Pages
- 6246-6252
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2101969937
- Cited by
- 250
- References
- 2
- FWCI
- 8.84
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Senga, T., Sivaprasad, U., Zhu, W., Park, J. H., Arias, E. E., Walter, J. C., & Dutta, A. (2006). PCNA Is a Cofactor for Cdt1 Degradation by CUL4/DDB1-mediated N-terminal Ubiquitination. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m512705200
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