Dynamic proteomics emphasizes the importance of selective mRNA translation and protein turnover during Arabidopsis seed germination.
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During seed germination, the transition from a quiescent metabolic state in a dry mature seed to a proliferative metabolic state in a vigorous seedling is crucial for plant propagation as well as for optimizing crop yield. This work provides a detailed description of the dynamics of protein synthesis during the time course of germination, demonstrating that mRNA translation is both sequential and selective during this process. The complete inhibition of the germination process in the presence of the translation inhibitor cycloheximide established that mRNA translation is critical for Arabidopsis seed germination. However, the dynamics of protein turnover and the selectivity of protein synthesis (mRNA translation) during Arabidopsis seed germination have not been addressed yet. Based on our detailed knowledge of the Arabidopsis seed proteome, we have deepened our understanding of seed mRNA translation during germination by combining two-dimensional gel-based proteomics with dynamic radiolabeled proteomics using a radiolabeled amino acid precursor, namely [(35)S]-methionine, in order to highlight de novo protein synthesis, stability, and turnover. Our data confirm that during early imbibition, the Arabidopsis translatome keeps reflecting an embryonic maturation program until a certain developmental checkpoint. Furthermore, by dividing the seed germination time lapse into discrete time windows, we highlight precise and specific patterns of protein synthesis. These data refine and deepen our knowledge of the three classical phases of seed germination based on seed water uptake during imbibition and reveal that selective mRNA translation is a key feature of seed germination. Beyond the quantitative control of translational activity, both the selectivity of mRNA translation and protein turnover appear as specific regulatory systems, critical for timing the molecular events leading to successful germination and seedling establishment.
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The imbibition period is crucial for seed germination, allowing activation of cellular metabolism including mobilization of food reserves and activation of mitochondria and protein synthesis.
“Since it provides the required water to hydrate the cellular constituents, the imbibition period is crucial for seed germination, allowing the activation of cellular metabolism which includes the mobilization of food reserves and the activation of mitochondria and protein synthesis (Galland et al., 2014; Paszkiewicz et al., 2017).”
From Exploring the Applicability of Calorespirometry to Assess Seed Metabolic Stability Upon Temperature Stress Conditions—Pisum sativum L. Used as a Case Study (2022) · cites this paper as Galland et al., 2014
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“their protein half-lives are short (129).”
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Rodrigues, L., Nogales, A., Hansen, L. D., Santos, F., Rato, A. E., & Cardoso, H. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.827117 · Cited by 8 · see citing works
- Authors
- Galland, M., Huguet, R., Arc, E., Cueff, G., Job, D., & Rajjou, L.
- Affiliations
- AgroParisTech (FR)
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Rajjou, Loïc
- Venue
- Molecular & Cellular Proteomics
- Publisher
- Elsevier BV
- Published
- 2014-01
- Volume
- 13
- Issue
- 1
- Pages
- 252-268
- ISSN
- 1535-9476 (ISSN-L)
- PMC
- PMC3879618
- OpenAlex
- W2147455540
- Cited by
- 184
- References
- 1
- Citations by year
- 2026: 12 · 2025: 20 · 2024: 13 · 2023: 21 · 2022: 10 · 2021: 17 · 2020: 16 · 2019: 15 · 2018: 15 · 2017: 10 · 2016: 13 · 2015: 14 · 2014: 7
- FWCI
- 13.69
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Molecular & Cellular Proteomics (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Seed Germination and Physiology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- seed germination, mRNA translation, protein turnover, Arabidopsis thaliana, translational regulation, cycloheximide, seed proteome, imbibition, de novo protein synthesis, seedling establishment
- MeSH terms
- Plant Proteins, RNA, Messenger, Seeds, Protein Biosynthesis, Arabidopsis, Gene Expression Regulation, Plant, Germination, Proteomics
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