The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Evaluation of Multiprotein Immunoaffinity Subtraction for Plasma Proteomics and Candidate Biomarker Discovery Using Mass Spectrometry
Open accessAbstract
Strategies for removal of high abundance proteins have been increasingly utilized in proteomic studies of serum/plasma and other body fluids to enhance the detection of low abundance proteins and achieve broader proteome coverage; however, both the reproducibility and specificity of the high abundance protein depletion process still represent common concerns. Here we report a detailed evaluation of immunoaffinity subtraction performed applying the ProteomeLab IgY-12 system that is commonly used in human serum/plasma proteome characterization in combination with high resolution LC-MS/MS. Plasma samples were repeatedly processed using this approach, and the resulting flow-through fractions and bound fractions were individually analyzed for comparison. The removal of target proteins by the immunoaffinity subtraction system and the overall process was highly reproducible. Non-target proteins, including one spiked protein standard (rabbit glyceraldehyde-3-phosphate dehydrogenase), were also observed to bind to the column at different levels but also in a reproducible manner. The results suggest that multiprotein immunoaffinity subtraction systems can be readily integrated into quantitative strategies to enhance detection of low abundance proteins in biomarker discovery studies.
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Cites
Anderson, N. L., & Anderson, N. G. (2002) · Molecular & Cellular Proteomics · doi:10.1074/mcp.r200007-mcp200 · Cited by 4,414
Pieper, R., Su, Q., Gatlin, C. L., Huang, S., Anderson, N. L., & Steiner, S. (2003) · PROTEOMICS · doi:10.1002/pmic.200390057 · Cited by 356
Cited by
Protein Analysis by Shotgun/Bottom-up Proteomics
Zhang, Y., Fonslow, B. R., Shan, B., Baek, M.-C., & Yates, J. R. (2013) · Chemical Reviews · doi:10.1021/cr3003533 · Cited by 1,491
Zhang, Q., Ames, J. M., Smith, R. D., Baynes, J. W., & Metz, T. O. (2009) · Journal of Proteome Research · doi:10.1021/pr800858h · Cited by 421
Self-citationDepletion of Abundant Plasma Proteins and Limitations of Plasma Proteomics
Tu, C., Rudnick, P. A., Martinez, M. Y., Cheek, K. L., Stein, S. E., Slebos, R. J. C., & Liebler, D. C. (2010) · Journal of Proteome Research · doi:10.1021/pr100646w · Cited by 390
Selected reaction monitoring applied to proteomics
Gallien, S., Duriez, E., & Domon, B. (2011) · Journal of Mass Spectrometry · doi:10.1002/jms.1895 · Cited by 286
Abbatiello, S. E., Schilling, B., Mani, D. R., Zimmerman, L. J., Hall, S. C., MacLean, B., Albertolle, M., Allen, S., Burgess, M., Cusack, M. P., et al. (2015) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m114.047050 · Cited by 215
Metadata
- Authors
- Liu, T., Qian, W.-J., Mottaz, H. M., Gritsenko, M. A., Norbeck, A. D., Moore, R. J., Purvine, S. O., Camp, D. G., & Smith, R. D.
- Venue
- Molecular & Cellular Proteomics
- Publisher
- Elsevier BV
- Published
- 2006-11
- Volume
- 5
- Issue
- 11
- Pages
- 2167-2174
- ISSN
- 1535-9476 (ISSN-L)
- OpenAlex
- W2125468516
- Cited by
- 208
- References
- 2
- FWCI
- 9.62
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Liu, T., Qian, W.-J., Mottaz, H. M., Gritsenko, M. A., Norbeck, A. D., Moore, R. J., Purvine, S. O., Camp, D. G., & Smith, R. D. (2006). Evaluation of Multiprotein Immunoaffinity Subtraction for Plasma Proteomics and Candidate Biomarker Discovery Using Mass Spectrometry. Molecular & Cellular Proteomics. https://doi.org/10.1074/mcp.t600039-mcp200
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