Molecular analysis of the pediatric ocular surface for fungi
Abstract
PURPOSE: To analyze the conjunctival flora of individuals 21 years of age or less for fungi using polymerase chain reaction (PCR) methodology. METHODS: Before povidone-iodine antisepsis, eye-swab specimens were collected from adolescent corneal donors preceding corneal excision and from children during preparation for strabismus surgery. Nucleic acid was extracted from the specimens and analyzed by PCR using primers designed for the detection of broad-spectrum fungal DNA and of Candida albicans-specific DNA. RESULTS: Twelve (38%) of 32 eye donor surfaces and 7 (23%) of 30 patient samples were positive for fungal DNA (P = 0.1). C. albicans DNA was detected in 6 (19%) of the decedents' eyes but from none of the surgical patients (P = 0.04). CONCLUSIONS: Fungi were present on the normal ocular surface of children and adolescents. C. albicans was more likely to be found postmortem than pre-surgically.
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Candida albicans is the most important fungal opportunistic pathogen.
“Candida albicans is the most important fungal opportunistic pathogen.”
From <i>Candida</i>Infections, Causes, Targets, and Resistance Mechanisms: Traditional and Alternative Antifungal Agents (2013) · cites this paper as [2]
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References 16 · Cited by 45
- Authors
- Wu, T. G., Mitchell, B. M., Carothers, T. S., Coats, D. K., Brady-McCreery, K. M., Paysse, E. A., & Wilhelmus, K. R.
- Affiliations
- Baylor College of Medicine (US)
- ORCID
- Paysse, Evelyn A.
- Venue
- Current Eye Research
- Publisher
- Informa UK Limited
- Published
- 2003-01
- Volume
- 26
- Issue
- 1
- Pages
- 33-36
- ISSN
- 0271-3683 (ISSN-L)
- 1460-2202
- OpenAlex
- W2150742950
- Cited by
- 45
- References
- 16
- Citations by year
- 2024: 1 · 2023: 5 · 2022: 8 · 2021: 4 · 2020: 1 · 2019: 5 · 2018: 3 · 2017: 2 · 2016: 2 · 2014: 1 · 2013: 1
- FWCI
- 0.69
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Current Eye Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Research to Prevent Blindness
- National Institutes of Health (5u10ey009696-05)
- National Eye Institute (EY09696, EY02520, P30EY002520, EY00377)
- Topics
- Health Sciences › Medicine › Ophthalmology › Ocular Infections and Treatments
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ocular Surface and Contact Lens
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Keywords
- conjunctival flora, Candida albicans, fungal DNA detection, corneal donors, strabismus surgery
- MeSH terms
- Adolescent, Adult, Aging, Candida albicans, Child, Child, Preschool, Conjunctiva, DNA, Fungal, Eye, Female, Fungi, Humans, Male, Tissue Donors, Single-Blind Method, Polymerase Chain Reaction
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