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Lectin histochemistry on the dorsal epidermis of the Breton dog

Desantis, Salvatore, Corriero, Aldo, Acone, Franca, Zubani, Dolores, Cirillo, Fedelia, Palmieri, Giovanni, De Metrio, Gregorio·2003·Acta Histochemica
DOI 10.1078/0065-1281-00686
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Abstract

Expression of sugar residues and the nature of oligosaccharide linkage during keratinocyte maturation in the epidermis of the Breton dog were studied with the use of lectin histochemistry. Thirteen lectins were used. Labelling was not observed with GSA I-B4, GSA II, UEA-I, and LTA. The cytoplasm of keratinocytes reacted with PNA, HPA, Con A, and WGA from the basal layer to the granular layer. PNA and Con A showed highest reactivity in the granular cell layer. The cell surface showed increased reactivity with PNA, HPA, and WGA with maturation of keratinocytes. KOH-neuraminidase treatment (KOH-Neu) increased PNA and RCA120 staining during keratinocyte differentiation thus indicating an increase in oligosaccharides terminating with sialic acid-Galbeta(1,3)GalNAc and sialic acid-Galbeta(1,4)GlcNAc, respectively. Labelling of the glycocalyx of basal and spinous keratinocytes with SNA and MAA revealed terminal Neu5acalpha(2,6)Gal/GalNAc and Neu5acalpha(2,3)Galbeta(1,4)GlcNAc. KOH-Neu-DBA showed oligosaccharides terminating with sialic acid-GalNAcalpha(1,3)GalNAc in the spinous and granular layers. A selective glycocalyx labelling of granular keratinocytes was observed with DBA and SBA. Reactions with MAA, PNA, DBA, RCA120, SBA, HPA, and WGA disappeared after the beta-elimination reaction. Our findings indicate that Breton dog epidermis contains more O-linked than N-linked oligosaccharides and confirm that different subpopulations of keratinocytes can be distinguished by lectin hist…

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Research works printed but not linked: 24

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Metadata

Authors
Desantis, S., Corriero, A., Acone, F., Zubani, D., Cirillo, F., Palmieri, G., & De Metrio, G.
Venue
Acta Histochemica
Publisher
Elsevier BV
Published
2003-01
Volume
105
Issue
1
Pages
73-79
ISSN
0065-1281
DOI
10.1078/0065-1281-00686
Cited by
9
References
0
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

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