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Purification and Characteristics of a Novel Transglutaminase Derived from Microorganisms

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Ando, Hiroyasu, Adachi, Masae, Umeda, Koichi, Matsuura, Akira, Nonaka, Masahiko, Uchio, Ryosuke, Tanaka, Haruo, Motoki, Masao·1989·Agricultural and Biological Chemistry

Abstract

A microorganism producing transglutaminase was screened as an indication of hydroxamate- forming activity. The microbial transglutaminase was purified from the culture filtrate of the strain, S- 8112, which was supposed to belong to the genus Streptoverticillium. The molecular weight of the purified enzyme was found to be about 40,000 on SDS-polyacrylamide gel electrophoresis, the isoelectric point 8.9 and the optimal pH of the reaction 6~7. The present enzyme requires no calcium ions for its activity. Thus, it clearly differs from known transglutaminases derived from mammalian organs, which have been defined as calcium-dependent enzymes.

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Metadata

Authors
Ando, H., Adachi, M., Umeda, K., Matsuura, A., Nonaka, M., Uchio, R., Tanaka, H., & Motoki, M.
Venue
Agricultural and Biological Chemistry
Published
1989-10-01
Volume
53
Issue
10
Pages
2613-2617
ISSN
0002-1369 (ISSN-L)
OpenAlex
W4248559484
Cited by
587
References
0
FWCI
1.91
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Purification and Characteristics of a Novel Transglutaminase Derived from Microorganisms. https://app.cochat.ai/papers/10.1080/00021369.1989.10869735
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Ando, H., Adachi, M., Umeda, K., Matsuura, A., Nonaka, M., Uchio, R., Tanaka, H., & Motoki, M. (1989). Purification and Characteristics of a Novel Transglutaminase Derived from Microorganisms. Agricultural and Biological Chemistry. https://doi.org/10.1080/00021369.1989.10869735

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.