CoChat
Sign Up
Cited by 260Stress Responses and CortisolClinical Laboratory Practices and Quality ControlAdrenal Hormones and Disorders

The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.

Long‐term stability of salivary cortisol

Garde, A. H., Hansen, Å. M.·2005·Scandinavian Journal of Clinical and Laboratory Investigation
DOI 10.1080/00365510510025773
Open this paper in CoChat — free account

Abstract

The measurement of salivary cortisol provides a simple, non-invasive, and stress-free measure frequently used in studies of the hypothalamic-pituitary-adrenal axis activity. In research projects, samples are often required to be stored for longer periods of time either because of the protocol of the project or because of lack of funding for analysis. The aim of the present study was to explore the effects of long-term storage of samples on the amounts of measurable cortisol. Ten pools of saliva were collected on polyester Salivette tampons from five subjects. After centrifugation the samples were either stored in small vials or spiked to polyester Salivette tampons before analysis for cortisol using Spectria RIA kits. The effects of storage were evaluated by a linear regression model (mixed procedure) on a logarithmic scale. No effects on cortisol concentrations were found after storage of saliva at 5 degrees C for up to 3 months or at -20 degrees C and -80 degrees C for up to one year. In contrast, concentrations of cortisol were found to decrease by 9.2% (95% confidence interval (CI): 3.8%; 14.3%) per month in samples stored at room temperature. Repeated freezing and thawing of samples up to four times before analysis did not affect the measured concentrations of cortisol. The coefficient of residual variation (CVresid) for samples stored on Salivette tampons were twice the CVresid for samples stored in separate vials after centrifugation. In conclusion, centrifuged saliva samples for analysis of cortisol may be stored at 5 degrees C for up to 3 months or at -20 degrees C or -80 degrees C for at least one year. However, long-term storage at room temperature cannot be recommended. Repeated cycles of freezing and thawing did not appear to affect the concentrations of cortisol.

Overview

Integrity signals

Registry

Crossref · 10.1080/00365510510025773

Cites

11 of 11 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 11 references

Research works printed but not linked: 1

  1. Aardal E., 1995, Eur J Clin Chem Clin Biochem, 33, 927

Cited by

Sorted by citations

Items 201–256 of 256

Related

See all

Metadata

Authors
Garde, A. H., & Hansen, Å. M.
Affiliations
Danish National Institute of Public Health (DK)
ORCID
Venue
Scandinavian Journal of Clinical and Laboratory Investigation
Publisher
Informa UK Limited
Published
2005-01
Volume
65
Issue
5
Pages
433-436
ISSN
  • 0036-5513 (ISSN-L)
  • 1502-7686
DOI
10.1080/00365510510025773
OpenAlex
W2064810136
Cited by
260
References
11
Citations by year
2026: 10 · 2025: 11 · 2024: 11 · 2023: 18 · 2022: 21 · 2021: 12 · 2020: 14 · 2019: 7 · 2018: 14 · 2017: 15 · 2016: 12 · 2015: 14 · 2014: 21 · 2013: 10 · 2012: 19
FWCI
3.12
Type
journal article
Language
en
Open access
closed
Locations
  • Scandinavian Journal of Clinical and Laboratory Investigation (publishedVersion)
  • PubMed (publishedVersion)
  • Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
Funders
Nordisk Ministerråd
Topics
  • Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
  • Health Sciences › Health Professions › Medical Laboratory Technology › Clinical Laboratory Practices and Quality Control
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
Keywords
salivary cortisol, sample storage stability, long-term storage, freeze-thaw cycles, room temperature storage, hypothalamic-pituitary-adrenal axis, cortisol measurement
MeSH terms
Freezing, Humans, Hydrocortisone, Saliva, Specimen Handling, Temperature

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review