Improvement of preservation method for ammonia-oxidizing bacteria by freeze-drying
Open accessAbstract
Long-term preservation of ammonia-oxidizing bacteria had been achieved until now by using the silica gel-freezing method. Recently, the freeze-drying method for the preservation of microorganisms has been widely used, and has been applied to the preservation of ammonia-oxidizing bacteria. The 10% skim milk-l% pyruvate or glutamate ratio was optimum as a dispersion-medium, and the preservation temperature of the freeze-dried cells was effective at -80°C. This method of preservation was found to be considerably more effective than the silica gel-freezing method.
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References 10 · Cited by 7
- Authors
- Satoh, K., Tanaka, T., Oguro, Y., Takahashi, R., & Tokuyama, T.
- Affiliations
- Nihon University (JP)
- ORCID
- Tanaka, Tomohiro
- Venue
- Soil Science and Plant Nutrition
- Publisher
- Informa UK Limited
- Published
- 2004-02
- Volume
- 50
- Issue
- 5
- Pages
- 777-781
- ISSN
- 0038-0768 (ISSN-L)
- 1747-0765
- OpenAlex
- W1977941173
- Cited by
- 7
- References
- 10
- Citations by year
- 2025: 1 · 2024: 1 · 2022: 2 · 2013: 1
- FWCI
- 0.68
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Soil Science & Plant Nutrition (publishedVersion)
- Funders
- Nihon University
- Ministry of Education, Culture, Sports, Science and Technology
- Topics
- Physical Sciences › Environmental Science › Pollution › Wastewater Treatment and Nitrogen Removal
- Life Sciences › Agricultural and Biological Sciences › Soil Science › Soil Carbon and Nitrogen Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Keywords
- ammonia-oxidizing bacteria, freeze-drying, microbial preservation, long-term preservation, skim milk, pyruvate, glutamate, dispersion medium, cryopreservation
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