Inhibition of Cancer Cell Replication by Inorganic Nitrite
Abstract
Nitrite has been implicated in carcinogenesis, especially under acidic conditions such as in the stomach or in urine, where it forms nitrosating species that can react with secondary amines to form nitrosamines. Recent studies have shown that nitrite and acid form a variety of other nitrogen oxides in vivo including nitric oxide-a compound with documented antitumor activity. Here we tested the effects of nitrite on bladder tumor cells incubated in mildly acidified urine. Nitrite (50 microM) inhibited thymidine incorporation in human T24 bladder cancer cells. This inhibition required slight acidification (pH 5.5-6), and no effect of nitrite could be observed at pH 7. Nitrite effects were further augmented in the presence of ascorbic acid, whereas ascorbic acid alone had no effect. The effects were paralleled by formation of nitric oxide gas. We here demonstrate an inhibitory effect of nitrite on cancer cell replication at concentrations and acidity commonly found in urine and gastric juice. The inhibitory effect is likely caused by nitric oxide and possibly other reactive nitrogen oxides formed from acidified nitrite.
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50 μM nitrite inhibited thymidine incorporation in human T24 bladder cancer cells under acidic conditions.
“Further work by the Lundberg group has confirmed this observation in a bladder tumor model such that 50 μM nitrite inhibited thymidine incorporation in human T24 bladder cancer cells under acidic conditions [263].”
From Inorganic nitrite therapy: historical perspective and future directions (2011) · cites this paper as [263]
Sodium nitrite administration to tumor bearing mice does not significantly affect growth rates.
“Conversely, it has been reported that sodium nitrite administration to tumor bearing mice does not significantly affect growth rates and that nitrite can inhibit cell division of certain tumor cell lines [263, 277].”
From Inorganic nitrite therapy: historical perspective and future directions (2011) · cites this paper as [263]
Nitrite can inhibit cell division of certain tumor cell lines.
“Conversely, it has been reported that sodium nitrite administration to tumor bearing mice does not significantly affect growth rates and that nitrite can inhibit cell division of certain tumor cell lines [263, 277].”
From Inorganic nitrite therapy: historical perspective and future directions (2011) · cites this paper as [263]
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References 35 · Cited by 17
- Authors
- Morcos, E., Carlsson, S., Weitzberg, E., Wiklund, N. P., & Lundberg, J. O.
- Affiliations
- Karolinska University Hospital (SE)
- Karolinska Institutet (SE)
- Venue
- Nutrition and Cancer
- Publisher
- Informa UK Limited
- Published
- 2010-04-28
- Volume
- 62
- Issue
- 4
- Pages
- 501-504
- ISSN
- 0163-5581 (ISSN-L)
- 1532-7914
- OpenAlex
- W2033971791
- Cited by
- 17
- References
- 35
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 1 · 2021: 1 · 2017: 2 · 2016: 1 · 2014: 3 · 2013: 1 · 2012: 2
- FWCI
- 0.91
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nutrition and Cancer (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer and biochemical research
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Keywords
- nitrite, nitric oxide, bladder cancer, T24 cells, acidification, vitamin C, reactive nitrogen oxides, nitrosamines, carcinogenesis, thymidine incorporation, urinary nitrite
- MeSH terms
- Antineoplastic Agents, Ascorbic Acid, Urinary Bladder Neoplasms, DNA Replication, Drug Synergism, Humans, Hydrogen-Ion Concentration, Luminescent Measurements, Nitric Oxide, Nitrites, Sodium Nitrite, Thymidine, Urine, Cell Line, Tumor
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