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Cited by 13Plant-Microbe Interactions and ImmunityAgriculture, Plant Science, Crop ManagementEntomopathogenic Microorganisms in Pest Control

The DOI matches its Crossref record.

Optimisation of a cost-effective culture medium for the large-scale production of Bacillus subtilis UTB96

Ghasemi, Soleiman, Ahmadzadeh, Masoud·2013·Archives Of Phytopathology And Plant Protection
DOI 10.1080/03235408.2013.771469
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Abstract

Fermentation optimisation to achieve high biomass and high efficiency of a biocontrol product is millstone in biocontrol sciences. Here, a Placket–Burman design used for comparison and screening of some environmental factors that are effective on bacterial biomass of Bacillus subtilis UTB96. A response surface methodology used to determine the optimal points for three factors including pH, temperature and C/N ratio where the biomass is high. The results of response surface methodology application showed that the optimum conditions for maximum production of biomass in the medium occurs at pH 7, temperature 30 °C and C/N ratio of 23:1. Bacteria derived from optimised conditions either from laboratory or semi-industrial bioreactors, showed a considerable increase in biomass and also, their antagonistic activity against Phytophthora drechsleri in a plate assay. However, optimisation of culture medium in a laboratory bioreactor decreased the antagonistic activity against Aspergillus flavus. Application of the optimised culture medium in both semi-industrial and laboratory bioreactors reduced the length of the lag phase of bacterial growth.

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Research works printed but not linked: 18

  1. Ashofteh F, 2006, J Plant Path, 91, 669
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Metadata

Authors
Ghasemi, S., & Ahmadzadeh, M.
Affiliations
University of Tehran (IR)
Corresponding author
Ahmadzadeh, Masoud
ORCID
Ahmadzadeh, Masoud
Venue
Archives Of Phytopathology And Plant Protection
Publisher
Informa UK Limited
Published
2013-08
Volume
46
Issue
13
Pages
1552-1563
ISSN
  • 0323-5408 (ISSN-L)
  • 1477-2906
DOI
10.1080/03235408.2013.771469
OpenAlex
W2086441823
Cited by
13
References
0
Citations by year
2026: 2 · 2025: 2 · 2024: 2 · 2023: 1 · 2020: 1 · 2019: 2 · 2018: 2 · 2016: 1
FWCI
0.40
Type
journal article
Language
en
Open access
closed
Locations
Archives of Phytopathology and Plant Protection (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Agriculture, Plant Science, Crop Management
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Entomopathogenic Microorganisms in Pest Control
Keywords
culture medium optimization, fermentation conditions, Plackett-Burman design, response surface methodology, C/N ratio, pH, temperature, bacterial biomass, biological control agents, Phytophthora drechsleri, Aspergillus flavus, antagonistic activity, lag phase

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