The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Platelet-released supernatants increase migration and proliferation, and decrease osteogenic differentiation of bone marrow-derived mesenchymal progenitor cells under in vitro conditions
Abstract
Platelet-rich plasma is currently promoted to serve as an adjuvant for bone grafts to enhance quantity and quality of newly forming bone; however, the underlying cellular mechanisms are not fully understood. We show here that supernatants of leukocyte-depleted thrombin-activated platelets increase migration and proliferation, and decrease osteogenic differentiation of bone marrow-derived mesenchymal progenitor cells under in vitro conditions. Using neutralizing antibodies raised against platelet-derived growth factor (PDGF), the observed effects of platelet-released supernatants were diminished. The mitogenic response was also decreased when extracellular signal-regulated protein kinase (ERK) signalling was inhibited by PD98059; however, PD98059 did not reverse the effects of platelet-released supernatants on migration and osteogenic differentiation. Consistent with an ERK-mediated mitogenic activity, incubation of serum-starved mesenchymal cell progenitors with platelet-released supernatants increased phosphorylation of the kinase. Together, these observations indicate that PDGF is a key factor released upon platelet activation that can increase migration and proliferation, and decreases osteogenic differentiation of mesenchymal progenitor cells under in vitro conditions. The results further suggest that ERK signalling is required to mediate the mitogenic response to platelet-released supernatants.
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Cites
Osteoclast differentiation and activation
Boyle, W. J., Simonet, W. S., & Lacey, D. L. (2003) · Nature · doi:10.1038/nature01658 · Cited by 6,670
Singer, A. J., & Clark, R. A. F. (1999) · New England Journal of Medicine · doi:10.1056/nejm199909023411006 · Cited by 6,106
Cited by
Fate decision of mesenchymal stem cells: adipocytes or osteoblasts?
Chen, Q., Shou, P., Zheng, C., Jiang, M., Cao, G., Yang, Q., Cao, J., Xie, N., Velletri, T., Zhang, X., et al. (2016) · Cell Death & Differentiation · doi:10.1038/cdd.2015.168 · Cited by 1,156
Isolation, cultivation, and characterization of human mesenchymal stem cells
Mushahary, D., Spittler, A., Kasper, C., Weber, V., & Charwat, V. (2018) · Cytometry Part A · doi:10.1002/cyto.a.23242 · Cited by 599
Ng, F., Boucher, S., Koh, S., Sastry, K. S. R., Chase, L., Lakshmipathy, U., Choong, C., Yang, Z., Vemuri, M. C., Rao, M. S., et al. (2008) · Blood · doi:10.1182/blood-2007-07-103697 · Cited by 580
Kocaoemer, A., Kern, S., Klüter, H., & Bieback, K. (2007) · Stem Cells · doi:10.1634/stemcells.2006-0627 · Cited by 414
Bone regeneration and stem cells
Arvidson, K., Abdallah, B. M., Applegate, L. A., Baldini, N., Cenni, E., Gomez-Barrena, E., Granchi, D., Kassem, M., Konttinen, Y. T., Mustafa, K., et al. (2011) · Journal of Cellular and Molecular Medicine · doi:10.1111/j.1582-4934.2010.01224.x · Cited by 408
Metadata
- Authors
- Gruber, R., Karreth, F., Kandler, B., Fuerst, G., Rot, A., Fischer, M. B., & Watzek, G.
- Venue
- Platelets
- Publisher
- Informa UK Limited
- Published
- 2004-02
- Volume
- 15
- Issue
- 1
- Pages
- 29-35
- ISSN
- 0953-7104 (ISSN-L)
- 1369-1635
- OpenAlex
- W2082882552
- Cited by
- 185
- References
- 2
- FWCI
- 10.18
- Type
- journal article
- Language
- en
- Open access
- closed
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- The paper
- Gruber, R., Karreth, F., Kandler, B., Fuerst, G., Rot, A., Fischer, M. B., & Watzek, G. (2004). Platelet-released supernatants increase migration and proliferation, and decrease osteogenic differentiation of bone marrow-derived mesenchymal progenitor cells under in vitro conditions. Platelets. https://doi.org/10.1080/09537100310001643999
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