Comparative transcriptome profiling of Kappaphycus alvarezii (Rhodophyta, Gigartinales) in response to two extreme temperature treatments: an RNA-seq-based resource for photosynthesis research
Open accessAbstract
Kappaphycus alvarezii provides an important raw material for the extraction of κ-carrageenan. Comparative transcriptome analysis of K. alvarezii after 24 h extreme temperature treatments (17°C and 34°C) was performed to uncover the molecular mechanisms controlling temperature-related photosynthesis regulation. Optimum temperature (28°C) was employed as the control. After de novo assembly of these three transcriptome samples and searching the assembled transcripts by BLAST against the NCBI nr databases, 11,537 top-hit transcripts were assigned as unigenes. Through comparisons, 302 significantly differentially expressed genes were identified. Twenty-three of the differentially expressed unigenes encoded photosystem antenna protein subunits and a further 25 were associated more broadly with photosynthesis functions. Compared to the light-harvesting complex (LHC) protein, the expression of phycobiliproteins (PBPs) was stable after extreme low and high temperature (ELT/EHT) treatments. Both ELT and EHT were found to inhibit the expression of subunits of photosystem II (PsbA, PsbC, Psb27, PsbW, PsbS), photosystem I (PsaF, PsaG, PsaK) and photosynthetic electron transport (PetE). These results were confirmed through targeted analysis of the mRNA and protein abundance of individual proteins, and were congruent with the results of chlorophyll a fluorescence (OJIP) measurements performed with temperature-stressed lines. In conclusion, similarities and differences in the behaviours of photosynthesis-related genes were summarized to provide opportunities for understanding the responses of K. alvarezii after extreme temperature treatments.
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The Handy PEA records chlorophyll fluorescence kinetics and allows detailed analysis of photosynthetic dynamics.
“Similarly, the Handy PEA from Hansatech Instruments records chlorophyll fluorescence kinetics and allows detailed analysis of photosynthetic dynamics [214].”
From Multi-Sensing Monitoring of the Microalgae Biomass Cultivation Systems for Biofuels and Added Value Products Synthesis—Challenges and Opportunities (2025) · cites this paper as [214]
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References 62 · Cited by 16
Research works printed but not linked: 1
- Dawes, C. J. (1989). Temperature acclimation in cultured Eucheuma isiforme from Florida and E. alvarezii from the Philippines. Journal of Applied Phycology 1: 59-65.
- Authors
- Li, Q., Zhang, L., Pang, T., & Liu, J.
- Affiliations
- Chinese Academy of Sciences (CN)
- Corresponding author
- Liu, Jianguo
- Venue
- European Journal of Phycology
- Publisher
- Informa UK Limited
- Published
- 2019-03-01
- Volume
- 54
- Issue
- 2
- Pages
- 162-174
- ISSN
- 0967-0262 (ISSN-L)
- 1469-4433
- OpenAlex
- W2919300881
- Cited by
- 16
- References
- 62
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 1 · 2023: 3 · 2021: 4
- FWCI
- 0.33
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- European Journal of Phycology (publishedVersion)
- Funders
- National Natural Science Foundation of China
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal plant biology
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Keywords
- Kappaphycus alvarezii, extreme temperature stress, photosynthesis regulation, photosystem II, photosystem I, phycobiliproteins, light-harvesting complexes, differentially expressed genes, transcriptome, photosynthetic electron transport
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