Acceleration of nitric oxide autoxidation and nitrosation by membranes
Open accessAbstract
The reaction between nitric oxide (•NO) and oxygen yields reactive species capable of oxidizing and nitrosating proteins, as well as deaminating DNA bases. Although this reaction is considered too slow to be biologically relevant, it has been shown that membranes, lipoproteins, mitochondria and possibly proteins can accelerate this reaction. This effect stems from the higher solubility of both •NO and O2in the hydrophobic phase of these biological particles, leading to a concentration of both reagents and so a higher rate of reaction. It has been determined that this reaction occurs from 30 to 300 times more rapidly within the membrane, while even higher values have been suggested for proteins. The autoxidation of •NO in membranes is not the main route for cellular •NO consumption but an important consequence of this phenomenon is to focus the generation of significant amounts of oxidizing and nitrosating molecules (nitrogen dioxide and dinitrogen trioxide) in the small volume comprised by cellular membranes. Even so, these reactive species are diffusible and their ultimate fate will depend on the reactivity towards available substrates rather than on physical barriers. The acceleration of •NO autoxidation by biological hydrophobic phases may thus be a general phenomenon that increases in importance in cases of •NO overproduction. IUBMB Life, 59: 243‐248, 2007
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Reaction of NO with O2 in the biological milieu is almost certainly not important as a source of nitrite formation compared with other much more rapid mechanisms.
“As we have speculated previously (41), reaction with O2 in the biological milieu is almost certainly not important as a source of nitrite formation compared with other much more rapid mechanisms (although it could conceivably be a localized source for reactive oxidative/nitrosative species, especially in hydrophobic environments).”
From What Part of NO Don't You Understand? Some Answers to the Cardinal Questions in Nitric Oxide Biology (2010) · cites this paper as [41]
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Cited by 122
Research works printed but not linked: 12
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- Lancaster, J. R., Jr. (2000) The physical properties of nitric oxide: determinants of the dynamics of NO in tissue. In Nitric Oxide: Biology and Pathobiology (Ignarro, L. J., ed.). pp. 209 -224, Academic Press, San Diego.
- Radi, R. (2004) Nitric oxide, oxidants, and protein tyrosine nitration. Proc. Natl. Acad. Sci. USA 101, 4003 -4008.
- Wright, M. M., Schopfer, F. J., Baker, P. R., Vidyasagar, V., Powell, P., Chumley, P., Iles, K. E., Freeman, B. A., and Agarwal, A. (2006) Fatty acid transduction of nitric oxide signaling: nitrolinoleic acid potently activates endothelial heme oxygenase 1 expression. Proc. Natl. Acad. Sci. USA 103, 4299 -4304.
- Schonhoff, C. M., Matsuoka, M., Tummala, H., Johnson, M. A., Estevez, A. G., Wu, R., Kamaid, A., Ricart, K. C., Hashimoto, Y., Gaston, B., Macdonald, T. L., Xu, Z., and Mannick, J. B. (2006) S-nitrosothiol depletion in amyotrophic lateral sclerosis. Proc. Natl. Acad. Sci. USA 103, 2404 -2409.
- Liu, X., Miller, M. J., Joshi, M. S., Thomas, D. D., and Lancaster, J. R., Jr. (1998) Accelerated reaction of nitric oxide with O 2 within the hydrophobic interior of biological membranes. Proc. Natl. Acad. Sci. USA 95, 2175 -2179.
- Shiva, S., Brookes, P. S., Patel, R. P., Anderson, P. G., and Darley- Usmar, V. M. (2001) Nitric oxide partitioning into mitochondrial membranes and the control of respiration at cytochrome c oxidase, Proc. Natl. Acad. Sci. USA 98, 7212 -7217.
- Mo¨ller, M. N., Li, Q., Vitturi, D. A., Robinson, J. M., Lancaster, J. R., Jr., and Denicola, A. (2007) The membrane 'lens' effect: focusing the formation of reactive nitrogen oxides from the NO/O 2 reaction. Chem. Res. Toxicol. 20(4), 709 -714.
- Rafikova, O., Rafikov, R., and Nudler, E. (2002) Catalysis of S-nitrosothiols formation by serum albumin: the mechanism and implication in vascular control. Proc. Natl. Acad. Sci. USA 99, 5913 - 5918.
- Shaw, A. W., and Vosper, A. J. (1976) Solubility of nitric oxide in aqueous and nonaqueous solvents. J. Chem. Soc. Faraday Trans. 18, 1239 -1244.
- Lakowicz, J. (1999) Protein fluorescence. In Principles of Fluorescence Spectroscopy (Lakowicz, J., ed.), 2nd edn, p. 463, Kluwer Academic/ Plenum Publishers, New York.
- Thomas, D. D., Liu, X., Kantrow, S. P., and Lancaster, J. R., Jr. (2001) The biological lifetime of nitric oxide: implications for the perivascular dynamics of NO and O2. Proc. Natl. Acad. Sci. USA 98, 355 -360.
- Authors
- Möller, M. N., Li, Q., Lancaster, J. R., & Denicola, A.
- Affiliations
- University of Alabama at Birmingham (US)
- Universidad de la República de Uruguay (UY)
- Venue
- IUBMB Life
- Publisher
- Wiley
- Published
- 2007-01
- Volume
- 59
- Issue
- 4-5
- Pages
- 243-248
- ISSN
- 1521-6543 (ISSN-L)
- 1521-6551
- OpenAlex
- W2070679513
- Cited by
- 122
- References
- 0
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 4 · 2023: 5 · 2022: 7 · 2021: 8 · 2020: 9 · 2019: 7 · 2018: 5 · 2017: 6 · 2016: 4 · 2015: 7 · 2014: 2 · 2013: 11 · 2012: 9
- FWCI
- 0.93
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- IUBMB Life (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Heart, Lung, and Blood Institute (HL 074391, R01HL074391)
- Fogarty International Center (R03TW007357, TW007357)
- Topics
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- biological membranes, reaction rate enhancement, lipoproteins, mitochondria, nitrogen dioxide, dinitrogen trioxide, protein oxidation, nitric oxide overproduction
- MeSH terms
- Animals, Cell Membrane, Nitric Oxide, Nitrites, Oxidation-Reduction, Oxygen, Proteins, Reactive Nitrogen Species
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