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Validation of metallothionein, interleukin-8, and heme oxygenase-1 as markers for the evaluation of cytotoxicity caused by metal oxide nanoparticles

Horie, Masanori, Shimizu, Kaori, Tabei, Yosuke·2018·Toxicology Mechanisms and Methods
DOI 10.1080/15376516.2018.1486931
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Abstract

Metal oxide nanoparticles have an industrial value, although their harmful effects are also known. Induction of respiratory inflammation through their inhalation is a serious indicator of their toxicity. Although the phenomenon of metal ion release is involved in the induction of inflammation, all metal ions are not necessarily toxic. However, currently, no particular index to evaluate cytotoxicity caused by nanoparticles exists. An index based on biological response is critical. In the present study, we examined the gene expression-based index for nanoparticle-derived cytotoxicity. The cellular effects of six kinds of metal oxide nanoparticles, ZnO, NiO, CuO, MgO, Bi 2 O 3 , and MoO 3 on A549 cells were examined. It was seen that lactate dehydrogenase (LDH) assay, which is one of the most important assays for assessing cell membrane damage, is inhibited by metal ions released from the metal oxide nanoparticles. In some cases, enzyme activity-based assay was not suitable for the evaluation of cytotoxicity of nanoparticles. ZnO and CuO nanoparticles displayed severe cytotoxicity and enhanced gene expression of heme oxygenase-1 (HO-1) and interleukin-8 (IL-8). The IL-8 gene expression was also increased from Bi 2 O 3 exposure. Additionally, the gene expression of metallothionein 2A (MT2A) was enhanced in the ZnO, CuO, and Bi 2 O 3 exposed cells. These results suggest that these nanoparticles released metal ions in the cells. The enhancement of HO-1, IL-8, and MT2A gene expressi…

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Metadata

Authors
Horie, M., Shimizu, K., & Tabei, Y.
Venue
Toxicology Mechanisms and Methods
Publisher
Informa UK Limited
Published
2018-08-15
Volume
28
Issue
8
Pages
630-638
ISSN
  • 1537-6516
  • 1537-6524
DOI
10.1080/15376516.2018.1486931
Cited by
22
References
0
Language
en

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CoChat. (2026). Citation check: Validation of metallothionein, interleukin-8, and heme oxygenase-1 as markers for the evaluation of cytotoxicity caused by metal oxide nanoparticles. https://app.cochat.ai/papers/10.1080/15376516.2018.1486931
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Horie, M., Shimizu, K., & Tabei, Y. (2018). Validation of metallothionein, interleukin-8, and heme oxygenase-1 as markers for the evaluation of cytotoxicity caused by metal oxide nanoparticles. Toxicology Mechanisms and Methods. https://doi.org/10.1080/15376516.2018.1486931