Genotypic variation in Penicillium chysogenum from indoor environments
Abstract
We examined 198 isolates of P. chysogenum recovered from 109 houses in Wallaceburg, Ontario, and 25 culture collection isolates including seven ex-type strains. Multilocus genotypes were determined by heteroduplex mobility assay of regions spanning introns in acetyl co-enzyme A synthase, beta-tubulin, thioredoxin reductase and the internal transcribed spacer regions of the nuclear ribosomal subrepeat. Five unique multilocus haplotypes were revealed without evidence of recombination, indicating strictly clonal population structures. Phylogenetic analysis of allele sequences using maximum parsimony resolved three strongly supported lineages. The dominant clade included more than 90% of house isolates in addition to the notable laboratory contaminant isolated by Alexander Fleming in 1929 in Britain. A second clade contained more than 5% of house isolates clustered with the ex-type strains of P. chysogenum and P. notatum. Follow-up sampling of outdoor air in the locality failed to reveal P. chysogenum, confirming the rarity of this fungus in outdoor air.
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Scott's lineages 1 and 2 are P. chrysogenum, and lineage 4 corresponds to P. rubens.
“Scott’s lineages 1 and 2 are P. chrysogenum, and lineage 4 corresponds to P. rubens.”
From Fleming’s penicillin producing strain is not Penicillium chrysogenum but P. rubens (2011) · cites this paper as Scott et al. 2004
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Fleming’s penicillin producing strain is not Penicillium chrysogenum but P. rubens
Houbraken, J., Frisvad, J. C., & Samson, R. A. (2011) · IMA Fungus · doi:10.5598/imafungus.2011.02.01.12 · Cited by 262 · see citing works
- Authors
- Scott, J., Untereiner, W. A., Wong, B., Straus, N. A., & Malloch, D.
- Affiliations
- University of Toronto (CA)
- Brandon University (CA)
- Corresponding author
- Scott, James
- ORCID
- Untereiner, Wendy A.
- Venue
- Mycologia
- Publisher
- Informa UK Limited
- Published
- 2004-09
- Volume
- 96
- Issue
- 5
- Pages
- 1095-1105
- ISSN
- 0027-5514 (ISSN-L)
- 1557-2536
- OpenAlex
- W7214206125
- Cited by
- 0
- References
- 0
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Mycologia (publishedVersion)
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Indoor Air Quality and Microbial Exposure
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Plant Pathogens and Fungal Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Keywords
- Penicillium chrysogenum, genotypic variation, multilocus genotypes, haplotypes, clonal population structure, indoor fungi, phylogenetic lineages, beta-tubulin, thioredoxin reductase, ITS region, ex-type strains, Penicillium notatum, fungal contamination
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