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Cited by 23Sperm and Testicular FunctionReproductive Biology and FertilityReproductive Health and Technologies

Early and late paternal effects of reactive oxygen species in semen on embryo development after intracytoplasmic sperm injection

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Kuroda, Shinnosuke, Takeshima, Teppei, Takeshima, Kazumi, Usui, Kimitsugu, Yasuda, Kengo, Sanjo, Hiroyuki, Kawahara, Takashi, Uemura, Hiroji +2 more·2020·Systems Biology in Reproductive Medicine
DOI 10.1080/19396368.2020.1720865
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Abstract

Although reactive oxygen species in semen are associated with unfavorable results with respect to assisted reproductive technology, their effects based on the detailed stages of embryo development are unclear. We investigated the relationship between reactive oxygen species in semen and the oocyte fertilization rate, cleavage rate, and blastulation rate of intracytoplasmic sperm injections. This retrospective study enrolled 77 couples who underwent intracytoplasmic sperm injection and analyzed 887 eggs from 141 cycles of intracytoplasmic sperm injection. The reactive oxygen species level in semen was compared between the fertilized and nonfertilized groups, between the good-cleavage-embryo and non-developed-embryo groups, and between the good-quality-blastocyst and poor-quality-blastocyst groups. The cut-off level of reactive oxygen species was calculated to predict good-cleavage-embryo and good-quality-blastocyst development. The fertilization rate was 65.4%, and the mean reactive oxygen species levels were not significantly different between the fertilized and nonfertilized groups. The reactive oxygen species level was significantly higher in the non-developed-embryo group than in the good-cleavage-embryo group (P = 0.0026) and was significantly lower in the good-quality-blastocyst group than in the poor-quality-embryo group (P = 0.015). Cleavage embryos and blastocysts were divided into high- and low-reactive-oxygen-species groups using a cut-off value of 6601 and 4926 relative light units, as calculated from the receiver operating characteristic curve. The rates of good-cleavage embryos and good-quality blastocysts were lower in the high-reactive-oxygen-species group than in the low-reactive-oxygen-species group, which were both statistically significant. To conclude, reactive oxygen species in semen is considered to have an adverse effect on both the early and late stages of embryo development in intracytoplasmic sperm injection.Abbreviations: GnRH, gonadotropin-releasing hormone; ICSI, intracytoplasmic sperm injection; IVF, in vitro fertilization; LPO, lipid peroxidation; NADPH, nicotinamide adenine dinucleotide phosphate; RLU, relative light units; ROC, receiver operating characteristic; ROS, reactive oxygen species

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  • Luminometric determination of seminal OS resulted in a significant prediction of good embryo cleavage and blastocyst quality after ICSI in the low OS group.

    “Similarly, the luminometric determination of seminal OS resulted in a significant prediction of good embryo cleavage and blastocyst quality after intracytoplasmic sperm injection (ICSI) in the low OS group [61].”

    From The Sixth Edition of the WHO Manual for Human Semen Analysis: A Critical Review and SWOT Analysis (2021) · cites this paper as [61]

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Cited by

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Authors
Kuroda, S., Takeshima, T., Takeshima, K., Usui, K., Yasuda, K., Sanjo, H., Kawahara, T., Uemura, H., Murase, M., & Yumura, Y.
Affiliations
Yokohama City University Medical Center (JP)
Corresponding author
Takeshima, Teppei
ORCID
Venue
Systems Biology in Reproductive Medicine
Publisher
Informa UK Limited
Published
2020-02-16
Volume
66
Issue
2
Pages
122-128
ISSN
  • 1939-6368 (ISSN-L)
  • 1939-6376
DOI
10.1080/19396368.2020.1720865
OpenAlex
W3005693191
Cited by
23
References
0
Citations by year
2026: 4 · 2025: 3 · 2024: 4 · 2023: 5 · 2022: 3 · 2021: 3 · 2020: 1
FWCI
2.65
Type
journal article
Language
en
Open access
Locations
  • Systems Biology in Reproductive Medicine (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
  • Health Sciences › Medicine › Reproductive Medicine › Reproductive Health and Technologies
Keywords
reactive oxygen species, semen, intracytoplasmic sperm injection, embryonic development, paternal effects, fertilization rate, cleavage rate, blastocyst quality, embryo quality prediction, oxidative stress
MeSH terms
Paternal Inheritance, Adult, Blastocyst, Cleavage Stage, Ovum, Female, Fertilization in Vitro, Humans, Male, Middle Aged, Pregnancy, Retrospective Studies, ROC Curve, Semen, Treatment Outcome, Reactive Oxygen Species, Sperm Injections, Intracytoplasmic, Embryonic Development

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