Butyrate mediates anti-inflammatory effects of Faecalibacterium prausnitzii in intestinal epithelial cells through Dact3
Open accessAbstract
The commensal bacterium Faecalibacterium prausnitzii plays a key role in inflammatory bowel disease (IBD) pathogenesis and serves as a general health biomarker in humans. However, the host molecular mechanisms that underlie its anti-inflammatory effects remain unknown. In this study we performed a transcriptomic approach on human intestinal epithelial cells (HT-29) stimulated with TNF-α and exposed to F. prausnitzii culture supernatant (SN) in order to determine the impact of this commensal bacterium on intestinal epithelial cells. Moreover, modulation of the most upregulated gene after F. prausnitzii SN contact was validated both in vitro and in vivo. Our results showed that F. prausnitzii SN upregulates the expression of Dact3, a gene linked to the Wnt/JNK pathway. Interestingly, when we silenced Dact3 expression, the effect of F. prausnitzii SN was lost. Butyrate was identified as the F. prausnitzii effector responsible for Dact3 modulation. Dact3 upregulation was also validated in vivo in both healthy and inflamed mice treated with either F. prausnitzii SN or the live bacteria, respectively. Finally, we demonstrated by colon transcriptomics that gut microbiota directly influences Dact3 expression. This study provides new clues about the host molecular mechanisms involved in the anti-inflammatory effects of the beneficial commensal bacterium F. prausnitzii.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Faecalibacterium preserves the gut-barrier function through the production of the SCFA butyrate and the secretion of anti-inflammatory mediators.
“There is evidence that Faecalibacterium preserves the gut-barrier function through the production of the SCFA butyrate and the secretion of anti-inflammatory mediators [57,58]”
From The Interplay between Gut Microbiota and Parkinson’s Disease: Implications on Diagnosis and Treatment (2022) · cites this paper as [58]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 45 · Cited by 245
- Authors
- Lenoir, M., Martín, R., Torres-Maravilla, E., Chadi, S., González-Dávila, P., Sokol, H., Langella, P., Chain, F., & Bermúdez-Humarán, L. G.
- Affiliations
- AgroParisTech (FR)
- Inserm (FR)
- Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France
- ORCID
- Venue
- Gut Microbes
- Publisher
- Informa UK Limited
- Published
- 2020-10-15
- Volume
- 12
- Issue
- 1
- Pages
- 1826748
- ISSN
- 1949-0976 (ISSN-L)
- 1949-0984
- PMC
- PMC7567499
- OpenAlex
- W3093230833
- Cited by
- 245
- References
- 45
- Citations by year
- 2026: 54 · 2025: 50 · 2024: 50 · 2023: 39 · 2022: 37 · 2021: 14 · 2017: 1
- FWCI
- 7.29
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Gut Microbes (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Vitagora Competitive Cluster
- French Fond Unique Interministériel (n°F1010012D)
- Fonds Européen de Développement Régional
- Merck Médication Familiale
- Biovitis
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Keywords
- Faecalibacterium prausnitzii, butyrate, intestinal epithelial cells, anti-inflammatory activity, inflammatory bowel disease, gut microbiota, TNF-α, HT-29 cells, commensal bacteria
- MeSH terms
- Gastrointestinal Microbiome, Faecalibacterium prausnitzii, Animals, Butyrates, Colon, Humans, Inflammation, Intestinal Mucosa, Male, Mice, Inbred C57BL, Tumor Necrosis Factor-alpha, Up-Regulation, Interleukin-8, HT29 Cells, Gene Expression Profiling, Adaptor Proteins, Signal Transducing, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
