Enhanced stability of microtubules enriched in detyrosinated tubulin is not a direct function of detyrosination level
Open accessAbstract
Interphase cultured monkey kidney (TC-7) cells contain distinct subsets of cellular microtubules (MTs) enriched in posttranslationally detyrosinated (Glu) or tyrosinated (Tyr) alpha tubulin (Gundersen, G. G., M. H. Kalnoski, and J. C. Bulinski. 1984. Cell. 38:779-789). To determine the relative stability of these subsets of MTs, we subjected TC-7 cells to treatments that slowly depolymerized MTs. We found Glu MTs to be more resistant than Tyr MTs to depolymerization by nocodazole in living cells, and to depolymerization by dilution in detergent-permeabilized cell models. However, in cold-treated cells, Glu and Tyr MTs did not differ significantly in their stability. Digestion of permeabilized cell models with pancreatic carboxypeptidase A, to generate Glu MTs from endogenous Tyr MTs, did not significantly alter the resistance of the endogenous Tyr MTs toward dilution-induced depolymerization. Furthermore, in human fibroblasts that contained no distinct Glu MTs, we observed a population of nocodazole-resistant MTs. These data suggest that Glu MTs possess enhanced stability against end-mediated depolymerization, yet detyrosination alone appears to be insufficient to confer this enhanced stability.
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Increasing MT detyrosination by VSH1/SVBP overexpression does not confer stability to microtubules; the majority of detyrosinated MTs are lost upon nocodazole treatment.
“we found that increasing MT detyrosination levels by overexpression of VSH1/SVBP does not confer stability to MTs as the majority of detyrosinated MTs were lost upon treatment with nocodazole (Fig. S1 G), in agreement with earlier work (Khawaja et al., 1988).”
From A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle (2023) · cites this paper as Khawaja et al., 1988
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Microtubules containing acetylated alpha-tubulin in mammalian cells in culture.
Piperno, G., LeDizet, M., & Chang, X. J. (1987) · The Journal of cell biology · doi:10.1083/jcb.104.2.289 · Cited by 873 · see citing works
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Cytoskeletal signaling: is memory encoded in microtubule lattices by CaMKII phosphorylation?
Craddock, T. J. A., Tuszynski, J. A., & Hameroff, S. (2012) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1002421 · Cited by 160 · see citing works
Merrick, S. E., Trojanowski, J. Q., & Lee, V. M.-Y. (1997) · The Journal of Neuroscience · doi:10.1523/jneurosci.17-15-05726.1997 · Cited by 143 · see citing works
Mechanisms of trafficking in axons and dendrites: implications for development and neurodegeneration
Sheetz, M. P., Pfister, K. K., Bulinski, J. C., & Cotman, C. W. (1998) · Progress in Neurobiology · doi:10.1016/s0301-0082(98)00021-5 · Cited by 55 · see citing works
Self-citationA live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle
Jansen, K. I., Iwanski, M. K., Burute, M., & Kapitein, L. C. (2023) · Journal of Cell Biology · doi:10.1083/jcb.202106105 · Cited by 39 · see citing works
- Authors
- Khawaja, S., Gundersen, G. G., & Bulinski, J. C.
- Affiliations
- University of California, Los Angeles (US)
- ORCID
- Gundersen, G G
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1988-01-01
- Volume
- 106
- Issue
- 1
- Pages
- 141-149
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2114950
- OpenAlex
- W2116170706
- Cited by
- 272
- References
- 1
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 3 · 2023: 10 · 2022: 9 · 2021: 7 · 2020: 4 · 2019: 3 · 2018: 5 · 2017: 7 · 2016: 5 · 2015: 7 · 2014: 6 · 2013: 4 · 2012: 9
- FWCI
- 7.95
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Cell Biology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (CA39755)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Health Sciences › Medicine › Oncology › Cancer Treatment and Pharmacology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
- Keywords
- microtubule stability, detyrosinated tubulin, alpha-tubulin, post-translational modification, nocodazole resistance, microtubule depolymerization, carboxypeptidase A, human fibroblasts, monkey kidney cells
- MeSH terms
- Animals, Benzimidazoles, Chlorocebus aethiops, Cold Temperature, Cytoskeleton, Fluorescent Antibody Technique, Glutamates, Microtubules, Polymers, Protein Binding, Solubility, Tubulin, Tyrosine, Nocodazole
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