The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Transforming growth factor-beta and the initiation of chondrogenesis and osteogenesis in the rat femur.
Open accessAbstract
We have investigated the ability of exogenous transforming growth factor-beta (TGF-beta) to induce osteogenesis and chondrogenesis, critical events in both bone formation and fracture healing. Daily injections of TGF-beta 1 or 2 into the subperiosteal region of newborn rat femurs resulted in localized intramembranous bone formation and chondrogenesis. After cessation of the injections, endochondral ossification occurred, resulting in replacement of cartilage with bone. Gene expression of type II collagen and immunolocalization of types I and II collagen were detected within the TGF-beta-induced cartilage and bone. Moreover, injection of TGF-beta 2 stimulated synthesis of TGF-beta 1 in chondrocytes and osteoblasts within the newly induced bone and cartilage, suggesting positive autoregulation of TGF-beta. TGF-beta 2 was more active in vivo than TGF-beta 1, stimulating formation of a mass that was on the average 375% larger at a comparable dose (p less than 0.001). With either TGF-beta isoform, the dose of the growth factor determined which type of tissue formed, so that the ratio of cartilage formation to intramembranous bone formation decreased as the dose was lowered. For TGF-beta 1, reducing the daily dose from 200 to 20 ng decreased the cartilage/intramembranous bone formation ratio from 3.57 to zero (p less than 0.001). With TGF-beta 2, the same dose change decreased the ratio from 3.71 to 0.28 (p less than 0.001). These data demonstrate that mesenchymal precursor cells in the periosteum are stimulated by TGF-beta to proliferate and differentiate, as occurs in embryologic bone formation and early fracture healing.
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Roberts, A. B., Sporn, M. B., Assoian, R. K., Smith, J. M., Roche, N. S., Wakefield, L. M., Heine, U. I., Liotta, L. A., Falanga, V, & Kehrl, J. H. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.12.4167 · Cited by 2,866
Self-citationDerynck, R., Jarrett, J. A., Chen, E. Y., Eaton, D. H., Bell, J. R., Assoian, R. K., Roberts, A. B., Sporn, M. B., & Goeddel, D. V. (1985) · Nature · doi:10.1038/316701a0 · Cited by 1,667
Self-citationAssoian, R. K., Komoriya, A., Meyers, C. A., Miller, D. M., & Sporn, M. B. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)32345-7 · Cited by 1,598
Self-citationKehrl, J. H., Wakefield, L. M., Roberts, A. B., Jakowlew, S., Alvarez-Mon, M., Derynck, R., Sporn, M. B., & Fauci, A. S. (1986) · The Journal of experimental medicine · doi:10.1084/jem.163.5.1037 · Cited by 1,582
Self-citationTransforming growth factor type beta induces monocyte chemotaxis and growth factor production.
Wahl, S. M., Hunt, D. A., Wakefield, L. M., McCartney-Francis, N., Wahl, L. M., Roberts, A. B., & Sporn, M. B. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.16.5788 · Cited by 1,272
Self-citationWound Macrophages Express TGF-α and Other Growth Factors in Vivo: Analysis by mRNA Phenotyping
Rappolee, D. A., Mark, D., Banda, M. J., & Werb, Z. (1988) · Science · doi:10.1126/science.3041594 · Cited by 1,104
Peptide growth factors are multifunctional
Sporn, M. B., & Roberts, A. B. (1988) · Nature · doi:10.1038/332217a0 · Cited by 818
Self-citationRole of transforming growth factor-beta in the development of the mouse embryo.
Heine, U., Munoz, E. F., Flanders, K. C., Ellingsworth, L. R., Lam, H. Y., Thompson, N. L., Roberts, A. B., & Sporn, M. B. (1987) · The Journal of cell biology · doi:10.1083/jcb.105.6.2861 · Cited by 745
Self-citationThe transforming growth factor-β system, a complex pattern of cross-reactive ligands and receptors
Cheifetz, S., Weatherbee, J. A., Tsang, M. L.-S., Anderson, J. K., Mole, J. E., Lucas, R., & Massagué, J. (1987) · Cell · doi:10.1016/0092-8674(87)90192-9 · Cited by 706
Centrella, M., McCarthy, T. L., & Canalis, E. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)61587-x · Cited by 611
Cited by
Barry, F., Boynton, R. E., Liu, B., & Murphy, J. M. (2001) · Experimental Cell Research · doi:10.1006/excr.2001.5278 · Cited by 1,020
Enhancement of fracture-healing.
Einhorn, T. A. (1995) · The Journal of Bone & Joint Surgery · doi:10.2106/00004623-199506000-00016 · Cited by 772
Carano, R. A. D., & Filvaroff, E. H. (2003) · Drug Discovery Today · doi:10.1016/s1359-6446(03)02866-6 · Cited by 746
Bulpitt, P., & Aeschlimann, D. (1999) · Journal of Biomedical Materials Research · doi:10.1002/(sici)1097-4636(199911)47:2<152::aid-jbm5>3.0.co;2-i · Cited by 560
In vivo engineering of organs: The bone bioreactor
Stevens, M. M., Marini, R. P., Schaefer, D., Aronson, J., Langer, R., & Shastri, V. P. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0504705102 · Cited by 339
Metadata
- Authors
- Joyce, M. E., Roberts, A. B., Sporn, M. B., & Bolander, M. E.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1990-06-01
- Volume
- 110
- Issue
- 6
- Pages
- 2195-2207
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2111019735
- Cited by
- 733
- References
- 41
- FWCI
- 13.10
- Type
- journal article
- Language
- en
- Open access
- green
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- Joyce, M. E., Roberts, A. B., Sporn, M. B., & Bolander, M. E. (1990). Transforming growth factor-beta and the initiation of chondrogenesis and osteogenesis in the rat femur. The Journal of cell biology. https://doi.org/10.1083/jcb.110.6.2195
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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