The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Low viscosity in the aqueous domain of cell cytoplasm measured by picosecond polarization microfluorimetry.
Open accessAbstract
Information about the rheological characteristics of the aqueous cytoplasm can be provided by analysis of the rotational motion of small polar molecules introduced into the cell. To determine fluid-phase cytoplasmic viscosity in intact cells, a polarization microscope was constructed for measurement of picosecond anisotropy decay of fluorescent probes in the cell cytoplasm. We found that the rotational correlation time (tc) of the probes, 2,7-bis-(2-carboxyethyl)-5-(and-6-)carboxyfluorescein (BCECF), 6-carboxyfluorescein, and 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) provided a direct measure of fluid-phase cytoplasmic viscosity that was independent of probe binding. In quiescent Swiss 3T3 fibroblasts, tc values were 20-40% longer than those in water, indicating that the fluid-phase cytoplasm is only 1.2-1.4 times as viscous as water. The activation energy of fluid-phase cytoplasmic viscosity was 4 kcal/mol, which is similar to that of water. Fluid-phase cytoplasmic viscosity was altered by less than 10% upon addition of sucrose to decrease cell volume, cytochalasin B to disrupt cell cytoskeleton, and vasopressin to activate phospholipase C. Nucleoplasmic and peripheral cytoplasmic viscosities were not different. Our results establish a novel method to measure fluid-phase cytoplasmic viscosity, and indicate that fluid-phase cytoplasmic viscosity in fibroblasts is similar to that of free water.
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Citations
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Molecular Rotor Measures Viscosity of Live Cells via Fluorescence Lifetime Imaging
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Metadata
- Authors
- Fushimi, K., & Verkman, A. S.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1991-02-15
- Volume
- 112
- Issue
- 4
- Pages
- 719-725
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2116043125
- Cited by
- 255
- References
- 23
- FWCI
- 8.37
- Type
- journal article
- Language
- en
- Open access
- green
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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