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Association of p60c-src with endosomal membranes in mammalian fibroblasts.
Open accessAbstract
We have examined the subcellular localization of p60c-src in mammalian fibroblasts. Analysis of indirect immunofluorescence by three-dimensional optical sectioning microscopy revealed a granular cytoplasmic staining that co-localized with the microtubule organizing center. Immunofluorescence experiments with antibodies against a number of membrane markers demonstrated a striking co-localization between p60c-src and the cation-dependent mannose-6-phosphate receptor (CI-MPR), a marker that identifies endosomes. Both p60c-src and the CI-MPR were found to cluster at the spindle poles throughout mitosis. In addition, treatment of interphase and mitotic cells with brefeldin A resulted in a clustering of p60c-src and CI-MPR at a peri-centriolar position. Biochemical fractionation of cellular membranes showed that a major proportion of p60c-src co-enriched with endocytic membranes. Treatment of membranes containing HRP to alter their apparent density also altered the density of p60c-src-containing membranes. Similar density shift experiments with total cellular membranes revealed that the majority of membrane-associated p60c-src in the cell is associated with endosomes, while very little is associated with plasma membranes. These results support a role for p60c-src in the regulation of endosomal membranes and protein trafficking.
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Cited by
du Manoir, S., Speicher, M. R., Joos, S., Schröck, E., Popp, S., Döhner, H., Kovacs, G., Robert-Nicoud, M., Lichter, P., & Cremer, T. (1993) · Human Genetics · doi:10.1007/bf00202476 · Cited by 555
Rho signaling, ROCK and mDia1, in transformation, metastasis and invasion
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Osteopetrosis in Src-deficient mice is due to an autonomous defect of osteoclasts.
Lowe, C., Yoneda, T., Boyce, B. F., Chen, H., Mundy, G. R., & Soriano, P. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.10.4485 · Cited by 329
Src family protein tyrosine kinases and cellular signal transduction pathways
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Metadata
- Authors
- Kaplan, K. B., Swedlow, J. R., Varmus, H. E., & Morgan, D. O.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1992-07-15
- Volume
- 118
- Issue
- 2
- Pages
- 321-333
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2063107082
- Cited by
- 233
- References
- 0
- FWCI
- 6.05
- Type
- journal article
- Language
- en
- Open access
- green
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- Kaplan, K. B., Swedlow, J. R., Varmus, H. E., & Morgan, D. O. (1992). Association of p60c-src with endosomal membranes in mammalian fibroblasts. The Journal of cell biology. https://doi.org/10.1083/jcb.118.2.321
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
