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FIXATION OF NEURAL TISSUES FOR ELECTRON MICROSCOPY BY PERFUSION WITH SOLUTIONS OF OSMIUM TETROXIDE
Open accessAbstract
This paper describes in detail a method for obtaining nearly uniform fixation of the nervous system by vascular perfusion with solutions of osmium tetroxide. Criteria are given for evaluating the degree of success achieved in the preservation of all the cellular components of the nervous system. The method permits analysis of the structural relations between cells at the electron microscopic level to an extent that has not been possible heretofore.
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Citations
Cited by
Diffusion in Brain Extracellular Space
Syková, E., & Nicholson, C. (2008) · Physiological Reviews · doi:10.1152/physrev.00027.2007 · Cited by 1,481
Development of local circuits in human visual cortex
Burkhalter, A., Bernardo, K., & Charles, V (1993) · The Journal of Neuroscience · doi:10.1523/jneurosci.13-05-01916.1993 · Cited by 219
Electron microscopy of oligodendrocytes in normal rat cerebrum
Kruger, L., & Maxwell, D. S. (1966) · American Journal of Anatomy · doi:10.1002/aja.1001180207 · Cited by 167
Aldehyde-stabilized cryopreservation
Song, A., & Fahy, G. M. (2015) · Cryobiology · doi:10.1016/j.cryobiol.2015.09.003 · Cited by 42
Ma, D., Holmes, H. E., Cardoso, M. J., Modat, M., Harrison, I. F., Powell, N. M., O’Callaghan, J. M., Ismail, O., Johnson, R. A., O’Neill, M. J., et al. (2019) · Frontiers in Neuroscience · doi:10.3389/fnins.2019.00011 · Cited by 36
Metadata
- Authors
- Palay, S. L., McGee-Russell, S. M., Gordon, S., & Grillo, M. A.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1962-02-01
- Volume
- 12
- Issue
- 2
- Pages
- 385-410
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2029375622
- Cited by
- 533
- References
- 0
- FWCI
- 79.73
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: FIXATION OF NEURAL TISSUES FOR ELECTRON MICROSCOPY BY PERFUSION WITH SOLUTIONS OF OSMIUM TETROXIDE. https://app.cochat.ai/papers/10.1083/jcb.12.2.385
- The paper
- Palay, S. L., McGee-Russell, S. M., Gordon, S., & Grillo, M. A. (1962). FIXATION OF NEURAL TISSUES FOR ELECTRON MICROSCOPY BY PERFUSION WITH SOLUTIONS OF OSMIUM TETROXIDE. The Journal of Cell Biology. https://doi.org/10.1083/jcb.12.2.385
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
